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His Tag Nickel Affinity Chromatography Protocol PDF - Details

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Protocols » Listing Details
ID:1232
Title: His Tag Nickel Affinity Chromatography Protocol PDF - http://www.mnstate.edu/provost/His_TagNiChromatogProtocol.pdf
Description:His Tag Nickel Affinity Chromatography Protocol PDF. The Wallert and Provost Lab. Theory and Introduction: Ni-Affinity Chromatography uses the ability of His to bind nickel. Six histadine amino acids at the end of a protein (either N or C terminus) is known as a 6X His tag. Nickel is bound to an agarose bead by chelation using nitroloacetic acid (NTA) beads. Several companies produce these beads as His Tagged proteins are some of the most used affinity tags in today’s market.
Category:Chromatography Protocols
Link Owner:
Date Added:September 07, 2007 10:20:09 AM
Number Hits:577
 

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