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Protocol for Motility using VE-DIC Microscopy - Details

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Protocols » Listing Details
ID:1222
Title: Protocol for Motility using VE-DIC Microscopy - http://www.proweb.org/kinesin/Methods/VE-DIC.html
Description:Protocol for motility using VE-DIC microscopy. Materials required: Anti-GST (glutathione S-transferase) antibody, PB buffer =10 mM NaPO4 pH 7.2, EGTA, MgCl2, Clarified motor lysate, MTs or Axoneme-MTs,Mg·ATP, VALAP (1 Vasoline: 1 Lanolin: 1 Paraffin, heated gently until melted) and Maxell XR-S120 Black Magnetite Super-VHS tapes or comparable.
Category:Cell Biology and Cell Culture
Link Owner:
Date Added:September 07, 2007 06:31:03 AM
Number Hits:4
 

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