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Selection of Poly(A)+ RNA by Oligo(dT)-Cellulose Chromatography - Subscription Required - Details

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Protocols » Listing Details
ID:1210
Title: Selection of Poly(A)+ RNA by Oligo(dT)-Cellulose Chromatography - Subscription Required - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot4047
Description:Chromatography on oligo(dT) columns is the preferred method for large-scale purification (>25 µg) of poly(A)+ RNA extracted from mammalian cells. Typically, between 1% and 10% of the RNA applied to the oligo(dT) column is recovered as poly(A)+ RNA. Because the method can be frustratingly slow, it is not recommended for purification of poly(A)+ RNA from multiple samples. For this purpose, batch elution (Selection of Poly(A)+ RNA by Batch Chromatography) is the better choice.
Category:Chromatography Protocols: DNA RNA Affinity Chromatography
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Date Added:September 07, 2007 05:07:51 AM
Number Hits:46
 

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