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DNA Affinity Chromatography Using Gravity Flow - Subscription Required - Details

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Protocols » Listing Details
ID:1204
Title: DNA Affinity Chromatography Using Gravity Flow - Subscription Required - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4206
Description:DNA Affinity Chromatography, DNA affinity chromatography can be a low-tech method using gravity flow at 4°C, a disposable chromatography column, and DNA affinity resin prepared in the laboratory (see Preparation of a DNA Affinity Column). Include 10-20% glycerol and 0.025-0.1% NP-40 in the column buffers to suppress losses due to nonspecific adsorption of protein to surfaces. Load the protein in a buffer that is compatible with binding of the protein to its target site. Keith Brocklehurst et al
Category:Chromatography Protocols: DNA RNA Affinity Chromatography
Link Owner:
Date Added:September 07, 2007 04:57:49 AM
Number Hits:176
 

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