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S20 Purification of detergent-insoluble lipid rafts from cells and tissues. - Details

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Protocols » Listing Details
ID:1189
Title: S20 Purification of detergent-insoluble lipid rafts from cells and tissues. - http://www.axis-shield.com/densityhome/optiprep/S20.pdf
Description:Protocol for the isolation of the lipid-rich microdomains of the plasma membrane, notably caveolae and lipid rafts. Methods for the isolation of lipid rafts are based on the insolubility of these structures in the nonionic detergent TritonX-100. Either the intact cells are treated with a detergent-containing solution or a post-nuclear supernatant is prepared from a cell homogenate and then Triton X-100 is added to this supernatant.
Category:Cell Biology and Cell Culture
Link Owner:
Date Added:September 06, 2007 06:55:29 PM
Number Hits:14
 

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