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Analysis Of DNA Fragmentation Using The JAM Assay (Subscription Required) - Details

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Protocols » Listing Details
ID:1180
Title: Analysis Of DNA Fragmentation Using The JAM Assay (Subscription Required) - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4432
Description:Analysis of DNA Fragmentation Using the JAM Assay. By Shailaja Kasibhatla et al., The JAM assay is based on labeling nuclear DNA of cycling cells with [3H]thymidine and harvesting samples on glass fiber filters. Apoptosis will generate DNA fragments small enough to pass through the glass fiber filter, resulting in decreased radioactivity of the particular sample. Cell-mediated cytotoxicity or cell killing mediated by cytotoxic T lymphocytes (CTL) can also be measured by this technique.
Category:Cell Biology and Cell Culture: Apoptosis Protocols: Apoptosis Analysis
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Date Added:September 03, 2007 04:57:35 PM
Number Hits:9
 

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