Tricineâ€“SDS-PAGE Protocol and background. Nature. PDF file. Tricineâ€“SDS-PAGE is commonly used to separate proteins in the mass range 1â€“100 kDa. It is the preferred electrophoretic
system for the resolution of proteins smaller than 30 kDa. â€“SDS-PAGE is also used preferentially for doubled SDS-PAGE (dSDS-PAGE), a proteomic tool used to isolate
extremely hydrophobic proteins for mass spectrometric identification.