Competent Cells Protocols

Protocols and information related to competent cells.

Protocols » Competent Cells Protocols

Competent Cells Protocols Protocol Links Sort by: Hits | Alphabetical

Electro Competent Cells Protocol - http://hulab.tamu.edu/wiki/index.php/Electro_competent_cells

Protocol for electro competent cells. Preparation of Electro competent E. coli cells (per the BioRad directions). - [Read Electro Competent Cells Protocol]

Frozen Competent E. coli Preparation and Transformation Protocol - http://genetics.med.harvard.edu/~cepko/protocol/mike/F3.html

This is a very easy protocol which yields transformation efficiencies appropriate for routine cloning and transformation. - [Read Frozen Competent E. coli Preparation and Transformation Protocol]

Preparing Electrocompetent Cells Protocol - http://openwetware.org/wiki/Knight:Preparing_electrocompetent_cells

Protocol for preparing electrocompetent cells. - [Read Preparing Electrocompetent Cells Protocol]

High Efficiency FCC Preparation and Tx Protocol - http://genetics.med.harvard.edu/~cepko/protocol/mike/F4.html

This is an easy and straightforward protocol that gives efficiencies of 106 to 107 cfu/mg of plasmid DNA. A growth curve is required for each strain that is prepared.
- [Read High Efficiency FCC Preparation and Tx Protocol]