Vector Protocols

Protocols and information related to vectors. Including: Vector production and purification; viral vectors; retroviral vectors.

Protocols » Cloning Protocols » Vector Protocols

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Directional Cloning into Plasmid Vectors Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3919

Directional cloning requires that the plasmid vector be cleaved with two restriction enzymes that generate incompatible termini and that the fragment of DNA to be cloned carries termini that are compatible with those of the doubly cleaved vector. - [Read Directional Cloning into Plasmid Vectors Protocol]

AAV Vector Production and Purification Protocol - http://www.brc.riken.jp/lab/dna/rvd/SOP/AAV/AAVProtocol.pdf

Protocol is used to propagate and purify AAV vectors for experiments both in vitro and in vivo. Includes: Principles of the Triple Plasmid Transfection System; Plasmids; Transfection and Extraction of Virus; Purification of the AAV vector. - [Read AAV Vector Production and Purification Protocol]

Blunt-ended Cloning into Plasmid Vectors Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3921

Protocol for blunt-ended cloning into plasmid vectors. - [Read Blunt-ended Cloning into Plasmid Vectors Protocol]

Transfection/Retroviral Infection pCL-Eco+Gene of Interest into 293T Cells Infecting HC11 Cells - http://www.bcm.edu/rosenlab/protocols/retroviral.pdf

Protocol for the transfection/Retroviral Infection pCL-Eco+Gene of interest into 293T cells infecting HC11 cells. Includes: Split 293T cells for transfection; Transfect 293T cells; Split HC11 cells for infection; Infect target (HC11) cells. - [Read Transfection/Retroviral Infection pCL-Eco+Gene of Interest into 293T Cells Infecting HC11 Cells]

Adherent Cell Types Infection Protocol - http://www.med.umich.edu/vcore/protocols/RetroviralCellScreenInfection101705.pdf

Protocol for infection for adherent cell types. - [Read Adherent Cell Types Infection Protocol]

Cloning siRNA Duplexes into pSUPER or pSUPER Retro Vectors Protocol - http://www.flemingtonlab.com/Protocols/pSUPERbasedsiRNAs.pdf

Protocol for cloning siRNA duplexes into pSUPER or pSUPER retro vectors. - [Read Cloning siRNA Duplexes into pSUPER or pSUPER Retro Vectors Protocol]

Phosphatase Treatment of Linearized Vector Protocol - http://openwetware.org/wiki/Phosphatase_treatment_of_linearized_vector

To minimize self-ligated vector in your transformation, treat your linearized vector with a phosphatase to remove the 5' phosphates necessary for ligation. This should improve the percentage of colonies with inserts. - [Read Phosphatase Treatment of Linearized Vector Protocol]

Viral Vector Handling Information - http://www.stanford.edu/dept/EHS/prod/researchlab/bio/docs/Palmer_Bio_protocol.pdf

Information on how to handle viral vectors. Includes: Adenovirus; MoMLV retrovirus; MoMLV-based Retroviral Vectors: Base classification BSL-1. - [Read Viral Vector Handling Information]

Lineage Analysis Using Retroviral Vectors Protocol - http://genetics.med.harvard.edu/~cepko/lineage/

Protocol describes the use of replication-incompetent retroviral vectors for the analysis of lineal relationships in developing vertebrate tissues. - [Read Lineage Analysis Using Retroviral Vectors Protocol]

In Vivo Cloning with Mu Protocol - http://rothlab.ucdavis.edu/protocols/in-vitro-mu-cloning.html

Protocol for in vivo cloning with mu. - [Read In Vivo Cloning with Mu Protocol]