Mutagenesis Protocols

Protocols and information related to mutagenesis and site directed mutagenesis.

Protocols » Cloning Protocols » Mutagenesis Protocols

Mutagenesis Protocols Protocol Links Sort by: Hits | Alphabetical

Creating a Deletion by PCR Splicing Protocol - http://www.methods.info/Tips/mutagenesis/PCR_splicing.html

This approach can be used to delete a fragment of any lenth or to introduce point mutations into a DNA seqence. - [Read Creating a Deletion by PCR Splicing Protocol]

F2 Mutant Screen - Non "Clonal" Protocol - http://cobweb.dartmouth.edu/cgi-bin/cgiwrap/~ambros/protocol.cgi?id=27

Protocol for F2 mutant screen (non "clonal"). - [Read F2 Mutant Screen - Non "Clonal" Protocol]

Hydroxylamine Mutagenesis of Plasmid DNA Protocol - http://www-rcf.usc.edu/~forsburg/hydroxyl.html

Protocol for hydroxylamine mutagenesis of plasmid DNA is ideal for random mutagenesis of plasmid DNA which is then used in a plasmid shuffle or screen for ts mutants. - [Read Hydroxylamine Mutagenesis of Plasmid DNA Protocol]

In Vitro Mutagenesis Using Double-stranded DNA Templates: Selection of Mutants with DpnI - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3813

Protocol for in vitro mutagenesis using double-stranded DNA templates. Two oligonucleotides are used to prime DNA synthesis catalyzed by a high-fidelity thermostable polymerase on a denatured plasmid template. The two oligonucleotides both contain the desired mutation and occupy the same starting and ending positions on opposite strands of the plasmid DNA. - [Read In Vitro Mutagenesis Using Double-stranded DNA Templates: Selection of Mutants with DpnI]

Integrating Extrachromasomal Arrays into the C. elegans Chromosomes Protocol - http://cobweb.dartmouth.edu/cgi-bin/cgiwrap/~ambros/protocol.cgi?id=4

Protocol for integrating extrachromasomal arrays into the C. elegans chromosomes. - [Read Integrating Extrachromasomal Arrays into the C. elegans Chromosomes Protocol]

Mutagenic PCR Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4143

Protocol for mutagenic PCR. - [Read Mutagenic PCR Protocol]

Oligonucleotide-directed Mutagenesis by Elimination of a Unique Restriction Site (USE Mutagenesis) - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3742

Protocol for oligonucleotide-directed mutagenesis by elimination of a unique restriction site (USE mutagenesis). - [Read Oligonucleotide-directed Mutagenesis by Elimination of a Unique Restriction Site (USE Mutagenesis)]

Oligonucleotide-directed Mutagenesis of Single-stranded DNA Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3817

Protocool for oligonucleotide-directed mutagenesis of single-stranded DNA. - [Read Oligonucleotide-directed Mutagenesis of Single-stranded DNA Protocol]

Phosphorylating the 5' Termini of Oligonucleotides Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3880

Protocol for phosphorylating the 5' termini of oligonucleotides. - [Read Phosphorylating the 5' Termini of Oligonucleotides Protocol]

Rapid and Efficient Site-directed Mutagenesis by the Single-tube Megaprimer PCR Method Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3467

Protocol for rapid and efficient site-directed mutagenesis by the single-tube megaprimer PCR method. - [Read Rapid and Efficient Site-directed Mutagenesis by the Single-tube Megaprimer PCR Method Protocol]

Rapid PCR Site-Directed Mutagenesis Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4144

Protocol for rapid PCR site-directed mutagenesis. - [Read Rapid PCR Site-Directed Mutagenesis Protocol]

Site Directed Mutagenesis Protocol - http://openwetware.org/wiki/Knight:Site-directed_mutagenesis

Protocol for site directed mutagenesis. - [Read Site Directed Mutagenesis Protocol]

Site-specific Mutagenesis by Overlap Extension Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3468

Protocol for site-specific mutagenesis by overlap extension. - [Read Site-specific Mutagenesis by Overlap Extension Protocol]

Systematic Mutagenesis of E.coli K-12 MG1655 ORFs Protocol - http://www.genome.wisc.edu/functional/tnmutagenesis.htm

Protocol for systematic mutagenesis of e. coli K-12 MG 1655. - [Read Systematic Mutagenesis of E.coli K-12 MG1655 ORFs Protocol]

Transposon Mediated Mutagenesis Protocol - http://www.genome.wisc.edu/resources/protocols/tnmutationprotocol.htm

Protocol for transposon mediated mutagenesis. Includes: Amplify ORF from MG1655; Transposition reaction; Transformation; Picking; Verify mutation by Culture PCR; Confirm mutations by sequencing; Curing the temperature sensitive pKD46 plasmid. - [Read Transposon Mediated Mutagenesis Protocol]