Liquid Chromatography Protocols

Protocols and information relating to liquid chromatography.

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Configuration Column Construction Column Packing for Capillary Liquid Chromatography - http://cshprotocols.cshlp.org/cgi/content/abstract/2007/3/pdb.prot4578

Protocol for configuration, column construction, and column packing for a capillary liquid chromatography system. Protocol describes a procedure for adapting conventional HPLC systems to provide accurate low-flow rates (0.4-4 µl/min) and gradients required to operate slurry-packed capillary columns. A key component of this system is a commercial axial-beam longitudinal flow cell that can be fitted to several commercial UV detectors. - [Read Configuration Column Construction Column Packing for Capillary Liquid Chromatography]

Desalting of Peptides and Protein Mixtures by RP-HPLC Techniques Protocol - http://cshprotocols.cshlp.org/cgi/content/abstract/2006/28/pdb.prot4549

Protocol for desalting of peptides and protein mixtures by RP-HPLC techniques. The RP-HPLC technique can be used to "desalt" peptide or protein samples derived from extraction procedures, from chemical reactions such as reductive alkylation in the presence of urea or guanidine hydrochloride, citraconylation, iodination, or cyanogen bromide cleavage, or recovered from other chromatographic separation. - [Read Desalting of Peptides and Protein Mixtures by RP-HPLC Techniques Protocol]

Determination of Neopterin and Biopterin by Liquid Chromatography Protocol - http://www.natureprotocols.com/2006/10/13/determination_of_neopterin_and.php

Protocol for determination of neopterin and biopterin by liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) in rat and human plasma, cell extracts and tissue homogenates. - [Read Determination of Neopterin and Biopterin by Liquid Chromatography Protocol]

HPLC Column Care Protocols and Information - http://www.nestgrp.com/pdf/colcare.pdf

Protocols and information on general HPLC column care. Includes: Silica based columns and Polymer based columns. Protocols included: pH stability, Mechanical stability, Mobile phases (Eluents), Proper storage of HPLC columns, Equilibration time, Regeneration of a column, Regeneration of RP packings, Regeneration of NP (Normal Phase) packings, Regeneration of Ion Exchange Packings - [Read HPLC Column Care Protocols and Information]

HPLC Separation of Digested Proteins and Preparation Protocol - http://cshprotocols.cshlp.org/cgi/content/abstract/2006/30/pdb.prot4663

Protocol for HPLC separation of digested proteins and preparation for matrix-assisted laser desorption/ionization analysis. - [Read HPLC Separation of Digested Proteins and Preparation Protocol]

Packing Capillary Columns for RP-HPLC Protocol - http://cshprotocols.cshlp.org/cgi/content/abstract/2006/28/pdb.prot4546

Protocol describes a procedure for adapting conventional HPLC systems to provide accurate low-flow rates (0.4-4 µl/min) and gradients required to operate slurry-packed capillary columns. A key component of this system is a commercial axial-beam longitudinal flow cell that can be fitted to a number of commercial UV detectors. - [Read Packing Capillary Columns for RP-HPLC Protocol]

Packing Ceramic Hydroxyapatite Columns for High-Performance Liquid Chromatography Protocol - http://cshprotocols.cshlp.org/cgi/content/extract/2006/9/pdb.prot4267

Protocol describes the packing of ceramic hydroxyapatite for HPLC. - [Read Packing Ceramic Hydroxyapatite Columns for High-Performance Liquid Chromatography Protocol]

Protein Separation on Ceramic Hydroxyapatite Using High-Performance Liquid Chromatography Protocol - http://cshprotocols.cshlp.org/cgi/content/extract/2006/9/pdb.prot4268

Protocol describes the separation of proteins using ceramic hydroxyapatite and HPLC. - [Read Protein Separation on Ceramic Hydroxyapatite Using High-Performance Liquid Chromatography Protocol]

Purification of Peptides from Solid-Phase Peptide Synthesis with RP-HPLC Protocol - http://cshprotocols.cshlp.org/cgi/content/abstract/2006/28/pdb.prot4548

Protocol details the purification and analysis of many synthetic peptides of 2-65 amino acid residues. These peptides contain a number of ionizable or polar side chains, but do not contain secondary structural elements (such as ß-sheets) that favor supramolecular assembly. - [Read Purification of Peptides from Solid-Phase Peptide Synthesis with RP-HPLC Protocol]

Stable Isotope Labeling by Amino Acids in Cell Culture SILAC Protocol - http://www.natureprotocols.com/2007/01/11/a_practical_recipe_for_stable.php

Protocol for stable isotope labeling by amino acids in cell culture (SILAC). Protocol describes how to apply SILAC and the use of nano-scale liquid chromatography coupled to electrospray ionization mass spectrometry for protein identification and quantification. This procedure can be completed in 8 days. - [Read Stable Isotope Labeling by Amino Acids in Cell Culture SILAC Protocol]

Standard Chromatographic Conditions for RP-HPLC of Proteins Protocol - http://cshprotocols.cshlp.org/cgi/content/extract/2006/1/pdb.prot4200

Protocol describes how to perform a test separation using a set of protein standards that have been designed to evaluate column performance. - [Read Standard Chromatographic Conditions for RP-HPLC of Proteins Protocol]