Affinity Antibody Purification of Protein A/G Chromatography Antibody Purification (Antiserum or Ascites by Protein A/G Chromatography). Species and Type of Antibody Agarose
Rabbit IgG Protein A or Protein G
Mouse IgG1 Protein G
Mouse IgG2 Protein A or Protein G
Mouse IgG3 Protein G
Sheep IgG Protein G but binds weakly
Rat IgG Protein G but binds weakly
Guinea Pig IgG Protein A
Dog IgG Protein A
Goat IgG Protein G
Pig IgG Protein A
Hamster IgG Protein G.
ANTIBODY PURIFICATION by affinity chromatography ANTIBODY PURIFICATION by affinity chromatography. By Beth, Mullins Lab UCSF. To affinity purify antibodies, generate lots of E. coli lysate that contains your antigen. If the protein can stand freeze thawing, then go ahead and purify the protein from e. coli lysate and keep it frozen until you need to couple it to a CH-sepharose column.
Purification of Antibodies on an Antigen Column Protocol Immunoaffinity purification of antibodies is used to purify antigen-specific antibodies from a preparation of polyclonal antibodies. Such purification is commonly needed in the production of antipeptide antibodies, where it is used to concentrate the desired antibodies and separate them from those raised against carrier proteins. It is also used for the more general purpose of removing unwanted, nonspecific binding activity from polyclonal antibody preparations.