Chromatography Protocols

Protocols for chromatography including: DNA/RNA Affinity Chromatography, Immunoaffinity Chromatography, Thin Layer Chromatography (TLC), Size Exclusion Chromatography, Gas Chromatography, Ion Exchange Chromatography, and Affinity chromatography.

Protocols » Chromatography Protocols

Chromatography Protocols Categories

Affinity Chromatography Protocols (8)

Column Chromatography Protocols (4)

DNA RNA Affinity Chromatography (5)

Dye Ligand Affinity Chromatography Protocols (6)

Gas Chromatography Protocols (4)

Gel Filtration Chromatography Protocols (1)

Immobilized Metal Ion Affinity Chromatography Protocols (11)

Immunoaffinity Chromatography Protocols (10)

Ion Exchange Chromatography Protocols (9)

Liquid Chromatography Protocols (11)

Chromatography Protocols Protocol Links Sort by: Hits | Alphabetical

His Tag Nickel Affinity Chromatography Protocol PDF - http://www.mnstate.edu/provost/His_TagNiChromatogProtocol.pdf

His Tag Nickel Affinity Chromatography Protocol PDF. The Wallert and Provost Lab. Theory and Introduction: Ni-Affinity Chromatography uses the ability of His to bind nickel. Six histadine amino acids at the end of a protein (either N or C terminus) is known as a 6X His tag. Nickel is bound to an agarose bead by chelation using nitroloacetic acid (NTA) beads. Several companies produce these beads as His Tagged proteins are some of the most used affinity tags in todayâ€™s market. - [Read His Tag Nickel Affinity Chromatography Protocol PDF]

FPLC Protocol - http://users.path.ox.ac.uk/~ciu/Methods/Protein/fplc.html

FPLC Protocol. The FPLC consists of a pump and a column which will withstand high pressure so separations can be carried out relatively quickly. For a detailed description there is a FPLC system handbook which is particularly useful for trouble shooting. For use of individual columns follow the "instructions" (in the green folder) which accompany each one. Sir William Dunn School of Pathology, Oxford University. - [Read FPLC Protocol]

Affinity Chromatography Handbook - http://books.google.com/books?id=kUl4k55F-fIC&pg=PA169&lpg=PA169&dq=dna+affinity+chromatography+protocol&source=web&ots=A7TBT29Ejf&sig=XkdH0cM4tNPKdN7y9XN2BfNMeno#PPA79,M1

Affinity Chromatography Handbook. Google Books. - [Read Affinity Chromatography Handbook]

Preparation of DNA Affinity Resin - Subscription Required - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4205

This protocol describes the preparation of concatamerized oligonucleotides and their coupling to cyanogen-bromide-activated Sepharose. The procedure uses a commercially activated resin, which can be purchased as a lyophilized powder. Keith Brocklehurst et al. - [Read Preparation of DNA Affinity Resin - Subscription Required]

Purification by affinity chromatography of H1Â° RNA-binding - http://147.52.72.117/IJMM/2003/volume11/number4/509.pdf

Purification by affinity chromatography of H1Â° RNA-binding proteins from rat brain. MARIA SCATURRO et al. - [Read Purification by affinity chromatography of H1Â° RNA-binding]

Purification of Nuclear Factor DNA Recognition Site Affinity PDF - http://www.jbc.org/cgi/reprint/261/3/1398.pdf

Purification of Nuclear Factor DNA Recognition Site Affinity. PDF. - [Read Purification of Nuclear Factor DNA Recognition Site Affinity PDF]