Nucleus Protocols

The nucleus contains most of the cell's genetic material, organized as multiple long linear DNA molecules in complex with a large variety of proteins, such as histones, to form chromosomes. The genes within these chromosomes make up the cell's nuclear genome. The function of the nucleus is to maintain the integrity of these genes and to control the activities of the cell by regulating gene expression. Find here information and protocols relating to the nucleus.

Protocols » Cell Organelle Protocols » Nucleus Protocols

Nucleus Protocols Protocol Links Sort by: Hits | Alphabetical

Nucleolar Isolation Protocol - http://www.lamondlab.com/f7nucleolarprotocol.htm

Protocol for nuclear isolation. - [Read Nucleolar Isolation Protocol]

Chromatin Isolation by Small-Scale Biochemical Fractionation Protocol - http://www.lamondlab.com/pdf/chromatinisolation.pdf

Protocol for chromatin isolation by small-scale biochemical fractionation. - [Read Chromatin Isolation by Small-Scale Biochemical Fractionation Protocol]

Cajal Body Isolation Protocol - http://www.lamondlab.com/f7cbprotocol.htm

Cajal Body Isolation Protocol. Protocol includes: Sonication, Removal Nucleoi, Gradient One, Gradient two, Concentration and final enrichment of cajal bodies. Also includes: Making 2.55M sucrose stock and Analysis of the enriched Cajal body fraction. - [Read Cajal Body Isolation Protocol]

CHO Centrosome Prep Protocol - http://mitchison.med.harvard.edu/protocols/mt1.html

The key step is the lysis which solubilizes centrosomes away from nuclei by very low ionic strength lysis after treatment of cells with nocodazole and cytochalasin B. The released centrosomes are then centrifuged onto a Ficoll cushion (to avoid pelleting) and the interface between the lysate and the Ficoll is collected and the centrosomes are concentrated on a sucrose gradient. Fractions are assayed by spindown and double IF with 5051 serum and anti-tubulin and the pooled fractions are frozen... - [Read CHO Centrosome Prep Protocol]

Isolation of Interphase Nuclei from Paraffin Section for FISH, Hedley Technique - http://www.riedlab.nci.nih.gov/publications/2408.2950_Isolat_Interph_Nuc4Hed204.pdf

Protocol for the isolation of interphase nuclei from Paraffin Section for FISH using the Hedley Technique. - [Read Isolation of Interphase Nuclei from Paraffin Section for FISH, Hedley Technique]

Protocol for Nonradioactive In Situ Hybridization to Polytene Chromosomes With DIG-labeled DNA Prob - http://www.roche-applied-science.com/PROD_INF/MANUALS/InSitu/pdf/ISH_108-111.pdf

A simplified and efficient protocol for nonradioactive in situ hybridization to polytene chromosomes with a DIG-labeled DNA probe. Includes: Labeling the hybridization probe; Preparation and denaturation of polytene chromosomes from Drosophila, Chironomus, or other species; Hybridization and detection; - [Read Protocol for Nonradioactive In Situ Hybridization to Polytene Chromosomes With DIG-labeled DNA Prob]