Mitochondria generate most of the cell's supply of adenosine triphosphate (ATP). They are also involved in a range of other processes, such as cell signaling, cellular differentiation, apoptosis, as well as the control of the cell cycle and cell growth. Mitochondria also possess their own independent genome. Find here protocols and information relating to mitochondria.
Analysis of Mitochondrial Membrane Potential with the Sensitive Fluorescent Probe JC-1 The technique of JC-1 staining has been developed with the intent to detect DY in intact, viable cells. For this purpose JC-1 acts as a marker of mitochondrial activity, since the formation of J-aggregates, which give red emission, is reversible. Cells with high DY are those forming J-aggregates, thus showing high red fluorescence. On the other hand, cells with low DY are those in which JC-1 maintains (or re-acquire) monomeric form, thus showing only green fluorescence.
Flow Cytometric Analysis of Mitochondrial Transmembrane Potential ({Delta}{Psi}m) Protocol describes a method for evaluation of mitochondrial function using the fluorochrome CMXRos. CMXRos is sequestered by actively respiring mitochondria, but washed out when the mitochondrial membrane potential is lost. This analysis can be combined with the TUNEL technique or immunocytochemistry.
Microscopic Analysis of Mitochondrial Transmembrane Potential Protocol Protocol describes a method for the evaluation of mitochondrial function using the fluorochrome CMXRos. CMXRos is sequestered by actively respiring mitochondria, but washed out when the mitochondrial membrane potential is lost. This analysis can be combined with the TUNEL technique or immunocytochemistry.
