Bioinformatics, Protocols, DNA RNA Protein Proteomics
 |
|---|
You have to register before you can post on our forums or use our advanced features. Register Now! Its Free and Fast!
Already registered? Login now below.
Already registered and Forgot your password? Click below to recover it.
Join now - it's fast and free!
Molecular Station is THE largest network of researchers, scientists and science lovers anywhere!
Research is what I'm doing when I don't know what I'm doing. ~Wernher Von Braun
Protocols and information related to cell storing and cell freezing.
| Cryogenic Preservation and Storage of Animal Cells Protocol - http://www.corning.com/Lifesciences/technical_information/techDocs/Cell_freezing_protocol.pdf Cryogenic preservation (storage below -100°C) of cell cultures is widely used to maintain backups or reserves of cells without the associated effort and expense of feeding and caring for them. The success of the freezing process depends on four critical areas: Proper handling and gentle harvesting of the cultures; Correct use of the cryoprotective agent; A controlled rate of freezing; Storage under proper cryogenic conditions. - [Read Cryogenic Preservation and Storage of Animal Cells Protocol] |
| Cryopreservation of Mammalian Culture Cells: Preparation and Recovery of Samples Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4350 Protocol provides methods for cryofreezing and subsequent thawing of mammalian cells. Pre-confluent cells are trypsinized, pelleted, resuspended in freezing medium, and gradually frozen. When needed, frozen cells are thawed quickly under running tap water and transferred to growth medium. - [Read Cryopreservation of Mammalian Culture Cells: Preparation and Recovery of Samples Protocol] |
| Defrosting Eukaryotic Cells Frozen In Liquid N2 Protocol - http://www2.umdnj.edu/~geller/lab/thawing.html Protocol for the defrosting of eukaryotic cells frozen in liquid N2. - [Read Defrosting Eukaryotic Cells Frozen In Liquid N2 Protocol] |
| Freezing and Thawing Cells Protocol - http://pingu.salk.edu/~sefton/Hyper_protocols/freezeThaw.html Protocol for freezing and thawing cells. - [Read Freezing and Thawing Cells Protocol] |
| Freezing and Thawing of Mammalian Cell Lines Protocol - http://research.cm.utexas.edu/bkitto/Kittolabpage/Protocols/Immunology/CellFreeze.html For long term storage of myeloma cells, hybridoma cells, T cells, and other mammalian cell lines in liquid nitrogen, and restoring them in culture. - [Read Freezing and Thawing of Mammalian Cell Lines Protocol] |
| Long Term Lambda Phage Storage Protocol - http://humgen.wustl.edu/hdk_lab_manual/lambda/lambda6.html No special treatment is required to prepare a lysate for the active collection. The following procedure should be used for long-term storage of lambda clones in the archival collections. The phage are diluted in media containing 7% DMSO and frozen at -80 degrees C. - [Read Long Term Lambda Phage Storage Protocol] |
| Thawing & Freezing Protocols for Insect Cells (BV) - http://www.ebioscience.com/ebioscience/appls/BVC.htm Protocols for thawing and freezing insect cells (BV). - [Read Thawing & Freezing Protocols for Insect Cells (BV)] |
Send this page to a friend