Bioinformatics, Protocols, DNA RNA Protein Proteomics
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If it's green or wriggles, it's biology. If it stinks, it's chemistry. If it doesn't work, it's physics. ~Handy Guide to Science
Protocols and information related to cell lines.
| Passage Procedure for RAW 264.7 Cells - http://www.signaling-gateway.org/data/cgi-bin/ProtocolFile.cgi/afcs_PP00000159.pdf?pid=PP00000159 RAW 264.7 cells are a macrophage-like, Abelson leukemia virus transformed cell line derived from BALB/c mice. For routine maintenance in culture (passage), cells are seeded at a confluence of approximately 10% (1 x 106 and 3 x 106 cells in 100-mm and 150-mm plates, respectively) and grown to a confluence of approximately 80%. This procedure requires the cells to be split every two days. - [Read Passage Procedure for RAW 264.7 Cells] |
| Preparation of Frozen Stocks of RAW 264.7 Cells - http://www.signaling-gateway.org/data/cgi-bin/ProtocolFile.cgi/afcs_PP00000180.pdf?pid=PP00000180 Protocol for the preparation of frozen stocks of RAW 264.7 cells. Includes: Freezing procedure and reagents. - [Read Preparation of Frozen Stocks of RAW 264.7 Cells] |
| Thaw Procedure for RAW 264.7 Cells - http://www.signaling-gateway.org/data/cgi-bin/ProtocolFile.cgi/afcs_PP00000160.pdf?pid=PP00000160 Protocol for the thaw procedure for RAW 264.7 cells. Includes: Thaw procedure and reagents and materials. - [Read Thaw Procedure for RAW 264.7 Cells] |
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