Bioinformatics, Protocols, DNA RNA Protein Proteomics
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Protocols and information related to C.elegans staining.
| Antibody Addition and Detection for Staining Caenorhabditis elegans Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/23/pdb.prot4523 Extreme care should be used to identify and verify positive reactions, however, because cross-reactions are common. Counterstaining is essential for examining worms by immunofluorescence and is used to identify the exact cell in which an antigen appears. Methods for counterstaining include labeling all cells with a fluorescent dye that is specific for nucleic acids (e.g., DAPI or propidium iodide) and using GFP driven by tissue-specific promoters. - [Read Antibody Addition and Detection for Staining Caenorhabditis elegans Protocol] |
| Antibody Staining of Dissected Gonads Protocol - http://www.genetics.wustl.edu/tslab/Protocols/gonad_antibody.html Protocol for antibody staining of dissected gonads. - [Read Antibody Staining of Dissected Gonads Protocol] |
| DAPI Staining Protocol - http://www.genetics.wustl.edu/tslab/Protocols/dapi_staining.html Protocol for DAPI staining. - [Read DAPI Staining Protocol] |
| Double Dye Filling (using DiO and di-4 ANEPPS) Protocol - http://www.k-state.edu/hermanlab/protocols/double_dye_fill.htm Protocol for double dye filling (using DiO and di-4 ANEPPS). - [Read Double Dye Filling (using DiO and di-4 ANEPPS) Protocol] |
| Dye Filling to Stain Amphid and Phasmid Neurons Protocol - http://cobweb.dartmouth.edu/cgi-bin/cgiwrap/~ambros/protocol.cgi?id=9 Protocol for dye filling to stain amphid and phasmid neurons. - [Read Dye Filling to Stain Amphid and Phasmid Neurons Protocol] |
| Nematode Dye Filling Protocol - http://www.k-state.edu/hermanlab/protocols/nematode_dye_filling.htm Protocol for nematode dye filling. Includes: Thumb plate; Microfuge tube; 15 ml tube; Viewing: Big Scope and Dissecting Scope. - [Read Nematode Dye Filling Protocol] |
| PAR-1 Staining Procedure Protocol - http://www-biology.ucsd.edu/labs/aroian/protocols/PAR1-stain.htm Protocol for PAR-1 staining procedure. Includes fixation and staining. - [Read PAR-1 Staining Procedure Protocol] |
| Phalloidin Staining of C. elegans Worms Protocol - http://cobweb.dartmouth.edu/cgi-bin/cgiwrap/~ambros/protocol.cgi?id=8 Protocol for phalloidin staining of C. elegans worms. - [Read Phalloidin Staining of C. elegans Worms Protocol] |
| RUVKUN Antibody Staining Protocol - http://cobweb.dartmouth.edu/cgi-bin/cgiwrap/~ambros/protocol.cgi?id=11 Protocol for RUVKUN Antibody Staining. Includes: Fixation; To make solution; REDUCING DISULFIDES TO -SH; OXIDIZE -SH GROUPS TO -SO3; TO CHECK THAT WORMS ARE PERMEABLE TO MACROMOLECULES; ANTIBODY INCUBATIONS; PERMANENT SPRINGTIME MOUNTING. - [Read RUVKUN Antibody Staining Protocol] |
| Staining C. elegans for ß-galactosidase Protocol - http://cobweb.dartmouth.edu/cgi-bin/cgiwrap/~ambros/protocol.cgi?id=10 Protocol for staining C. elegans for ß-galactosidase. - [Read Staining C. elegans for ß-galactosidase Protocol] |
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