C. elegans Genetics Protocols

Protocol and information related to C. elegans genetics.

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C. elegans Genetics Protocols Protocol Links Sort by: Hits | Alphabetical

C. elegans EMS Mutagenesis Protocol - http://cobweb.dartmouth.edu/cgi-bin/cgiwrap/~ambros/protocol.cgi?id=26

Protocol for C. elegans EMS mutagenesis. - [Read C. elegans EMS Mutagenesis Protocol]

C. elegans Genomic DNA Prep Protocol - http://www.k-state.edu/hermanlab/protocols/genomic_dna_prep.htm

Protocol for C. elegans genomic DNA prep. - [Read C. elegans Genomic DNA Prep Protocol]

C. elegans RNAi Protocol - http://www.zoology.ubc.ca/~alorch/rnai-protocol.htm

Protocol for C. elegans RNAi. Includes: Transformation of competent cells; Blunt-end ligation; Preparation of competent cells; Dephosphorylation of linear plasmid DNA; Restriction Digest: EcoRV; Insert amplification from gDNA; Gel purification: QiaQuick gel purification kit; Mini-prep; Transformant Screening; Bacterial preparation and induction; Preparation of worms for RNAi feeding. - [Read C. elegans RNAi Protocol]

Single Worm PCR Protocol - http://www.genetics.wustl.edu/tslab/Protocols/Single_worm_PCR_v2.htm

Protocol for single worm PCR. - [Read Single Worm PCR Protocol]

C. elegans Integrating Extrachromasomal Arrays Protocol - http://cobweb.dartmouth.edu/cgi-bin/cgiwrap/~ambros/protocol.cgi?id=4

Protocol and information related to C. elegans integrating extrachromasomal arrays. - [Read C. elegans Integrating Extrachromasomal Arrays Protocol]

Guide to Genetic Mapping in C. elegans - http://www.uwyo.edu/MolecBio/Fay/WBF_-_Super_Geniuses.pdf

A guide to genetic mapping in C. elegans. Includes: Introduction and basics; Two-point mapping; Three-point mapping; Deficiency and duplication mapping; Basics of SNP mapping; Mapping dominant mutations; Mapping suppressor/enhance mutations; Mapping of synthetic mutations. - [Read Guide to Genetic Mapping in C. elegans]

EMS Mutagenesis of C. elegans Protocol - http://www.k-state.edu/hermanlab/protocols/ems_mutagenesis.htm

Protocol for EMS mutagenesis of C. elegans. - [Read EMS Mutagenesis of C. elegans Protocol]

C. elegans Integration via Gamma Radiation Protocol - http://www.biochem.northwestern.edu/ibis/morimoto/research/Protocols/IX.%20C.%20elegans/E.%20Genetics/1.%20Integration%20via%20gamma.pdf

Protocol for C. elegans integration via gamma radiation. - [Read C. elegans Integration via Gamma Radiation Protocol]

Introduction of Double-Stranded RNA in C. elegans by Feeding Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4317

Protocol describes an easily scalable way of introducing double-stranded RNA (dsRNA) in Caenorhabditis elegans: feeding the nematode with bacteria that express dsRNA. When using an RNase-III-negative Escherichia coli strain (HT115), the efficiency of this method is comparable to the alternative. - [Read Introduction of Double-Stranded RNA in C. elegans by Feeding Protocol]

C. elegans mRNA Prep Protocol - http://www.k-state.edu/hermanlab/protocols/joes_mrna_prep.htm

Protocol for C. elegans mRNA prep. Includes: Oligo-dT cellulose prep; mRNA prep. - [Read C. elegans mRNA Prep Protocol]

C. elegans Gene Knockout Protocol - http://www.zoology.ubc.ca/~alorch/koprotocol.htm

The procedure is to mutagenize a large population of worms with trimethylpsoralen and UV irradiation, set up 1152 subpopulations, screen DNA made from this library for deletions in specific genes by nested PCR, and then to recover single worms carrying the deletions through a sib-selection process. - [Read C. elegans Gene Knockout Protocol]

Preparation of C. elegans Genomic DNA Protocol - http://www.genetics.wustl.edu/tslab/Protocols/genomic_DNA_prep.htm

Protocol for preparation of C. elegans genomic DNA. Includes: Solutions; Worm preparation; DNA preparation. - [Read Preparation of C. elegans Genomic DNA Protocol]

C. elegans RNA Prep Protocol - http://www.k-state.edu/hermanlab/protocols/yale_rna_prep.htm

Protocol for C. elegans RNA prep. - [Read C. elegans RNA Prep Protocol]

C. elegans of F2 Mutant Screen - non "clonal" Protocol - http://cobweb.dartmouth.edu/cgi-bin/cgiwrap/~ambros/protocol.cgi?id=27

Protocol for C. elegans F2 mutant screen - non "clonal". - [Read C. elegans of F2 Mutant Screen - non "clonal" Protocol]

Isolation of RNA from C. elegans Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4321

Protocol describes an efficient approach to isolate RNA from Caenorhabditis elegans. - [Read Isolation of RNA from C. elegans Protocol]

Mapping C. elegans Mutants using STS Polymorphisms Protocol - http://cobweb.dartmouth.edu/cgi-bin/cgiwrap/~ambros/protocol.cgi?id=32

Protocol for mapping C. elegans mutants using STS polymorphisms. - [Read Mapping C. elegans Mutants using STS Polymorphisms Protocol]

C. elegans MMS Integration Protocol - http://www.k-state.edu/hermanlab/protocols/mms_integration.htm

Protocol for C. elegans MMS integration. Includes: Henderson Method and Koelle Method . - [Read C. elegans MMS Integration Protocol]

Introduction of Double-Stranded RNA in C. elegans by Soaking Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4316

Double-stranded RNA (dsRNA) can be introduced into Caenorhabditis elegans by soaking the animals in a solution of dsRNA. Alternative methods are dsRNA injection (see Introduction of Double-stranded RNA in C. elegans by Injection) and feeding the animals with bacteria that produce dsRNA. - [Read Introduction of Double-Stranded RNA in C. elegans by Soaking Protocol]

Introduction of Double-Stranded RNA in C. elegans by Injection Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4315

Double-stranded RNA (dsRNA) can be efficiently introduced into Caenorhabditis elegans by microinjection into the gonad, the gut, or the body fluid. The RNAi effect will spread within the nematode, exerting an effect beyond the site of injection. - [Read Introduction of Double-Stranded RNA in C. elegans by Injection Protocol]

Cosuppression in C. elegans Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4318

The cosuppression effect in C. elegans does not spread throughout the animal. Cosuppression in C. elegans can be triggered by highly repetitive transgenes that contain gene constructs. - [Read Cosuppression in C. elegans Protocol]