Bioinformatics, Protocols, DNA RNA Protein Proteomics
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I have had my results for a long time: but I do not yet know how I am to arrive at them. ~Karl Friedrich Gauss
Protocol and information related to C. elegans genetics.
| C. elegans EMS Mutagenesis Protocol - http://cobweb.dartmouth.edu/cgi-bin/cgiwrap/~ambros/protocol.cgi?id=26 Protocol for C. elegans EMS mutagenesis. - [Read C. elegans EMS Mutagenesis Protocol] |
| C. elegans Gene Knockout Protocol - http://www.zoology.ubc.ca/~alorch/koprotocol.htm The procedure is to mutagenize a large population of worms with trimethylpsoralen and UV irradiation, set up 1152 subpopulations, screen DNA made from this library for deletions in specific genes by nested PCR, and then to recover single worms carrying the deletions through a sib-selection process. - [Read C. elegans Gene Knockout Protocol] |
| C. elegans Genomic DNA Prep Protocol - http://www.k-state.edu/hermanlab/protocols/genomic_dna_prep.htm Protocol for C. elegans genomic DNA prep. - [Read C. elegans Genomic DNA Prep Protocol] |
| C. elegans Integrating Extrachromasomal Arrays Protocol - http://cobweb.dartmouth.edu/cgi-bin/cgiwrap/~ambros/protocol.cgi?id=4 Protocol and information related to C. elegans integrating extrachromasomal arrays. - [Read C. elegans Integrating Extrachromasomal Arrays Protocol] |
| C. elegans Integration via Gamma Radiation Protocol - http://www.biochem.northwestern.edu/ibis/morimoto/research/Protocols/IX.%20C.%20elegans/E.%20Genetics/1.%20Integration%20via%20gamma.pdf Protocol for C. elegans integration via gamma radiation. - [Read C. elegans Integration via Gamma Radiation Protocol] |
| C. elegans MMS Integration Protocol - http://www.k-state.edu/hermanlab/protocols/mms_integration.htm Protocol for C. elegans MMS integration. Includes: Henderson Method and Koelle Method . - [Read C. elegans MMS Integration Protocol] |
| C. elegans mRNA Prep Protocol - http://www.k-state.edu/hermanlab/protocols/joes_mrna_prep.htm Protocol for C. elegans mRNA prep. Includes: Oligo-dT cellulose prep; mRNA prep. - [Read C. elegans mRNA Prep Protocol] |
| C. elegans of F2 Mutant Screen - non "clonal" Protocol - http://cobweb.dartmouth.edu/cgi-bin/cgiwrap/~ambros/protocol.cgi?id=27 Protocol for C. elegans F2 mutant screen - non "clonal". - [Read C. elegans of F2 Mutant Screen - non "clonal" Protocol] |
| C. elegans RNA Prep Protocol - http://www.k-state.edu/hermanlab/protocols/yale_rna_prep.htm Protocol for C. elegans RNA prep. - [Read C. elegans RNA Prep Protocol] |
| C. elegans RNAi Protocol - http://www.zoology.ubc.ca/~alorch/rnai-protocol.htm Protocol for C. elegans RNAi. Includes: Transformation of competent cells; Blunt-end ligation; Preparation of competent cells; Dephosphorylation of linear plasmid DNA; Restriction Digest: EcoRV; Insert amplification from gDNA; Gel purification: QiaQuick gel purification kit; Mini-prep; Transformant Screening; Bacterial preparation and induction; Preparation of worms for RNAi feeding. - [Read C. elegans RNAi Protocol] |
| Cosuppression in C. elegans Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4318 The cosuppression effect in C. elegans does not spread throughout the animal. Cosuppression in C. elegans can be triggered by highly repetitive transgenes that contain gene constructs. - [Read Cosuppression in C. elegans Protocol] |
| EMS Mutagenesis of C. elegans Protocol - http://www.k-state.edu/hermanlab/protocols/ems_mutagenesis.htm Protocol for EMS mutagenesis of C. elegans. - [Read EMS Mutagenesis of C. elegans Protocol] |
| Guide to Genetic Mapping in C. elegans - http://www.uwyo.edu/MolecBio/Fay/WBF_-_Super_Geniuses.pdf A guide to genetic mapping in C. elegans. Includes: Introduction and basics; Two-point mapping; Three-point mapping; Deficiency and duplication mapping; Basics of SNP mapping; Mapping dominant mutations; Mapping suppressor/enhance mutations; Mapping of synthetic mutations. - [Read Guide to Genetic Mapping in C. elegans] |
| Introduction of Double-Stranded RNA in C. elegans by Feeding Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4317 Protocol describes an easily scalable way of introducing double-stranded RNA (dsRNA) in Caenorhabditis elegans: feeding the nematode with bacteria that express dsRNA. When using an RNase-III-negative Escherichia coli strain (HT115), the efficiency of this method is comparable to the alternative. - [Read Introduction of Double-Stranded RNA in C. elegans by Feeding Protocol] |
| Introduction of Double-Stranded RNA in C. elegans by Injection Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4315 Double-stranded RNA (dsRNA) can be efficiently introduced into Caenorhabditis elegans by microinjection into the gonad, the gut, or the body fluid. The RNAi effect will spread within the nematode, exerting an effect beyond the site of injection. - [Read Introduction of Double-Stranded RNA in C. elegans by Injection Protocol] |
| Introduction of Double-Stranded RNA in C. elegans by Soaking Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4316 Double-stranded RNA (dsRNA) can be introduced into Caenorhabditis elegans by soaking the animals in a solution of dsRNA. Alternative methods are dsRNA injection (see Introduction of Double-stranded RNA in C. elegans by Injection) and feeding the animals with bacteria that produce dsRNA. - [Read Introduction of Double-Stranded RNA in C. elegans by Soaking Protocol] |
| Isolation of RNA from C. elegans Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4321 Protocol describes an efficient approach to isolate RNA from Caenorhabditis elegans. - [Read Isolation of RNA from C. elegans Protocol] |
| Mapping C. elegans Mutants using STS Polymorphisms Protocol - http://cobweb.dartmouth.edu/cgi-bin/cgiwrap/~ambros/protocol.cgi?id=32 Protocol for mapping C. elegans mutants using STS polymorphisms. - [Read Mapping C. elegans Mutants using STS Polymorphisms Protocol] |
| Preparation of C. elegans Genomic DNA Protocol - http://www.genetics.wustl.edu/tslab/Protocols/genomic_DNA_prep.htm Protocol for preparation of C. elegans genomic DNA. Includes: Solutions; Worm preparation; DNA preparation. - [Read Preparation of C. elegans Genomic DNA Protocol] |
| Single Worm PCR Protocol - http://www.genetics.wustl.edu/tslab/Protocols/Single_worm_PCR_v2.htm Protocol for single worm PCR. - [Read Single Worm PCR Protocol] |
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