Bioinformatics, Protocols, DNA RNA Protein Proteomics
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Protocols and information related to C. elegans fixing.
| C. elegans Gonad Fixation Protocol - http://www.genetics.wustl.edu/tslab/Protocols/gonad_fixation.html Protocol for C.elegans gonad fixation. Includes fixation with: Methanol, Formaldehyde, Methanol / Formadehyde, Glutaraldehyde / Formaldehyde. - [Read C. elegans Gonad Fixation Protocol] |
| EM Fixation of C. elegans Embryos Protocol - http://www.aecom.yu.edu/wormem/embryo.htm Protocol for EM fixation of C. elegans embryos. - [Read EM Fixation of C. elegans Embryos Protocol] |
| Fixation and Permeabilization of C. elegans Protocol - http://cobweb.dartmouth.edu/cgi-bin/cgiwrap/~ambros/protocol.cgi?id=35 Protocol for fixation and permeabilization of C. elegans. Protocol works for all stages except dauers (which won't open) and hypoclorite-treated eggs (which disintegrate). Luckily, hypochlorite treatment and fixation are by themselves sufficient to open eggs. - [Read Fixation and Permeabilization of C. elegans Protocol] |
| Fixation and Staining Parameters - http://www-biology.ucsd.edu/labs/aroian/protocols/fixatives.htm Parameters for fixation and staining. Includes: Antigen, Fixation, Fixation time, Antibody and Dilution. - [Read Fixation and Staining Parameters] |
| Fixing Caenorhabditis elegans in Bouin’s Fixative Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/23/pdb.prot4521 Bouin’s fixative is a particularly good choice for worms because it penetrates dense tissues well and is extremely good for fixing antigens. Like all strong fixatives, however, it is unsuitable for some antibody-antigen pairs. In such cases, the length of time in the Bouin’s fixative can be shortened, or paraformaldehyde fixation can be used instead. - [Read Fixing Caenorhabditis elegans in Bouin’s Fixative Protocol] |
| Fixing Caenorhabditis elegans in Paraformaldehyde Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/23/pdb.prot4522 Common method for fixing worms is to use paraformaldehyde. This method provides a gentler fixation than the Bouin’s method, but often requires the use of collagenase. This method is particularly good for examining adult worms. - [Read Fixing Caenorhabditis elegans in Paraformaldehyde Protocol] |
| Two Step Fixation for C. elegans Protocol - http://www.aecom.yu.edu/wormem/micro.htm Protocol for two step fixation for C. elegans. - [Read Two Step Fixation for C. elegans Protocol] |
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