C. elegans Cell Culture Protocols

Protocol describes how to seed plates with E. coli OP50 for C. elegans cultures. Includes: Bacterial Stock Plate Preparation; Bacterial Broth Preparation; Seeding Bacteria Plates for Culturing Worms. - [Read Preparation of Seeded NGM Plates For Worm Food Protocol]

Protocol for liquid culture of worms. Includes: superbroth; S- basal; worm plates; GROWING THE BACTERIA (WORM FOOD); GROWING THE WORMS; AFTER THE CULTURE HAS GROWN; PREPARING EGGS TO START SYNCHRONIZED LIQUID CULTURES. - [Read Liquid Culture of Worms Protocol]

Protocol for cleaning worms by floating on sucrose. - [Read Cleaning Worms by Floating on Sucrose Protocol]

Protocol for freezing worms. Includes: The preferred method and The agar method. - [Read Freezing Worms Protocol]

Protocol for the development of techniques for primary culture of C. elegans embryonic neurons. - [Read Development of Techniques for Primary Culture of C. elegans Embryonic Neurons]

Protocol for cleaning worm stocks. - [Read Cleaning Worm Stocks Protocol]

Protocol for Dauer pheromone prep. Includes small molecule prep and cleaner prep. - [Read Dauer Pheromone Prep Protocol]

Protocol for lactose RNAi plates: minimal lactose media. - [Read Lactose RNAi Plates: Minimal Lactose Media Protocol]

Protocol for liquid culture of worms with fermentor. - [Read Liquid Culture of Worms with Fermentor Protocol]

Development of techniques for primary culture of C. elegans embryonic neurons. Includes: Optimized cell culture protocol. - [Read Development of Techniques for Primary Culture of C. elegans Embryonic Neurons 2nd Part]

Protocol for making Dauers with dauer pheromone. - [Read Making Dauers with Dauer Pheromone Protocol]

Development of techniques for primary culture of C. elegans embryonic neurons. - [Read Development of Techniques for Primary Culture of C. elegans Embryonic Neurons 3rd Part]