C. elegans Cell Culture Protocols

Cleaning Worm Stocks Protocol Protocol for cleaning worm stocks.

Cleaning Worms by Floating on Sucrose Protocol Protocol for cleaning worms by floating on sucrose.

Dauer Pheromone Prep Protocol Protocol for Dauer pheromone prep. Includes small molecule prep and cleaner prep.

Development of Techniques for Primary Culture of C. elegans Embryonic Neurons Protocol for the development of techniques for primary culture of C. elegans embryonic neurons.

Development of Techniques for Primary Culture of C. elegans Embryonic Neurons 2nd Part Development of techniques for primary culture of C. elegans embryonic neurons. Includes: Optimized cell culture protocol.

Development of Techniques for Primary Culture of C. elegans Embryonic Neurons 3rd Part Development of techniques for primary culture of C. elegans embryonic neurons.

Freezing Worms Protocol Protocol for freezing worms. Includes: The preferred method and The agar method.

Lactose RNAi Plates: Minimal Lactose Media Protocol Protocol for lactose RNAi plates: minimal lactose media.

Liquid Culture of Worms Protocol Protocol for liquid culture of worms. Includes: superbroth; S- basal; worm plates; GROWING THE BACTERIA (WORM FOOD); GROWING THE WORMS; AFTER THE CULTURE HAS GROWN; PREPARING EGGS TO START SYNCHRONIZED LIQUID CULTURES.

Liquid Culture of Worms with Fermentor Protocol Protocol for liquid culture of worms with fermentor.

Making Dauers with Dauer Pheromone Protocol Protocol for making Dauers with dauer pheromone.

Preparation of Seeded NGM Plates For Worm Food Protocol Protocol describes how to seed plates with E. coli OP50 for C. elegans cultures. Includes: Bacterial Stock Plate Preparation; Bacterial Broth Preparation; Seeding Bacteria Plates for Culturing Worms.