Bioinformatics, Protocols, DNA RNA Protein Proteomics
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Louise- "How did you get here?" Johnny- "Well, basically, there was this little dot, right? And the dot went bang and the bang expanded. Energy formed into matter, matter cooled, matter lived, the amoeba to fish, to fish to fowl, to fowl to frog, to frog to mammal, the mammal to monkey, to monkey to man, amo amas amat, quid pro quo, memento mori, ad infinitum, sprinkle on a little bit of grated cheese and leave under the grill till Doomsday." ~From the movie Naked
Protocols and information related to C. elegans cell culture.
| Cleaning Worm Stocks Protocol - http://cobweb.dartmouth.edu/cgi-bin/cgiwrap/~ambros/protocol.cgi?id=19 Protocol for cleaning worm stocks. - [Read Cleaning Worm Stocks Protocol] |
| Cleaning Worms by Floating on Sucrose Protocol - http://cobweb.dartmouth.edu/cgi-bin/cgiwrap/~ambros/protocol.cgi?id=18 Protocol for cleaning worms by floating on sucrose. - [Read Cleaning Worms by Floating on Sucrose Protocol] |
| Dauer Pheromone Prep Protocol - http://cobweb.dartmouth.edu/cgi-bin/cgiwrap/~ambros/protocol.cgi?id=12 Protocol for Dauer pheromone prep. Includes small molecule prep and cleaner prep. - [Read Dauer Pheromone Prep Protocol] |
| Development of Techniques for Primary Culture of C. elegans Embryonic Neurons - http://cobweb.dartmouth.edu/cgi-bin/cgiwrap/~ambros/protocol.cgi?id=14 Protocol for the development of techniques for primary culture of C. elegans embryonic neurons. - [Read Development of Techniques for Primary Culture of C. elegans Embryonic Neurons] |
| Development of Techniques for Primary Culture of C. elegans Embryonic Neurons 2nd Part - http://cobweb.dartmouth.edu/cgi-bin/cgiwrap/~ambros/protocol.cgi?id=15 Development of techniques for primary culture of C. elegans embryonic neurons. Includes: Optimized cell culture protocol. - [Read Development of Techniques for Primary Culture of C. elegans Embryonic Neurons 2nd Part] |
| Development of Techniques for Primary Culture of C. elegans Embryonic Neurons 3rd Part - http://cobweb.dartmouth.edu/cgi-bin/cgiwrap/~ambros/protocol.cgi?id=16 Development of techniques for primary culture of C. elegans embryonic neurons. - [Read Development of Techniques for Primary Culture of C. elegans Embryonic Neurons 3rd Part] |
| Freezing Worms Protocol - http://cobweb.dartmouth.edu/cgi-bin/cgiwrap/~ambros/protocol.cgi?id=20 Protocol for freezing worms. Includes: The preferred method and The agar method. - [Read Freezing Worms Protocol] |
| Lactose RNAi Plates: Minimal Lactose Media Protocol - http://www.genetics.wustl.edu/tslab/Protocols/Lactose_RNAi_Plates.htm Protocol for lactose RNAi plates: minimal lactose media. - [Read Lactose RNAi Plates: Minimal Lactose Media Protocol] |
| Liquid Culture of Worms Protocol - http://cobweb.dartmouth.edu/cgi-bin/cgiwrap/~ambros/protocol.cgi?id=22 Protocol for liquid culture of worms. Includes: superbroth; S- basal; worm plates; GROWING THE BACTERIA (WORM FOOD); GROWING THE WORMS; AFTER THE CULTURE HAS GROWN; PREPARING EGGS TO START SYNCHRONIZED LIQUID CULTURES. - [Read Liquid Culture of Worms Protocol] |
| Liquid Culture of Worms with Fermentor Protocol - http://cobweb.dartmouth.edu/cgi-bin/cgiwrap/~ambros/protocol.cgi?id=21 Protocol for liquid culture of worms with fermentor. - [Read Liquid Culture of Worms with Fermentor Protocol] |
| Making Dauers with Dauer Pheromone Protocol - http://cobweb.dartmouth.edu/cgi-bin/cgiwrap/~ambros/protocol.cgi?id=13 Protocol for making Dauers with dauer pheromone. - [Read Making Dauers with Dauer Pheromone Protocol] |
| Preparation of Seeded NGM Plates For Worm Food Protocol - http://www.bio.com/protocolstools/protocol.jhtml?id=p1558 Protocol describes how to seed plates with E. coli OP50 for C. elegans cultures. Includes: Bacterial Stock Plate Preparation; Bacterial Broth Preparation; Seeding Bacteria Plates for Culturing Worms. - [Read Preparation of Seeded NGM Plates For Worm Food Protocol] |
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