Bioinformatics, Protocols, DNA RNA Protein Proteomics
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Life is short, the art long. ~Hippocrates c.460 - 357 BC. Greek Physician and the Father of Medicine.
Protocols and information related to C.elegans. Including: C. Elegans Fixing; C. elegans Genetics; C. elegans Injection; C. elegans Nucleic Acids Related; C. elegans Staining; C. elegans culture.
C. elegans Cell Culture Protocols (12)C. elegans Fixing Protocols (7)C. elegans Genetics Protocols (20) | C. elegans Injection Protocols (3)C. elegans Staining Protocols (10) |
| Bacteria-Mediated (Feeding) RNAi Protocol - http://www.genetics.wustl.edu/tslab/Protocols/Feeding_RNAi.htm Protocol for bacteria- mediated (feeding) RNAi. - [Read Bacteria-Mediated (Feeding) RNAi Protocol] |
| Cytosolic Extract & Immunoprecipitation Protocol - http://www.genetics.wustl.edu/tslab/Protocols/CytosolicExtractv1_v2.htm Protocol for cytosolic extract & immunoprecipitation. Includes: Culture; Harvest; Extract; Immunoprecipitation. - [Read Cytosolic Extract & Immunoprecipitation Protocol] |
| Gonad Dissections Protocol - http://www.genetics.wustl.edu/tslab/Protocols/gonad_dissections.html Protocol for gonad dissections. - [Read Gonad Dissections Protocol] |
| Immunofluorescence of C. elegans Embryos Protocol - http://www-biology.ucsd.edu/labs/aroian/protocols/immunofluorescence.htm Protocol for immunofluorescence of C. elegans embryos. - [Read Immunofluorescence of C. elegans Embryos Protocol] |
| Immunofluorescence using Anti-Serotonin Antibody in C. elegans Protocol - http://www.bio.com/protocolstools/protocol.jhtml?id=p1561 Protocol for immunofluorescence using anti-serotonin antibody in C. elegans. - [Read Immunofluorescence using Anti-Serotonin Antibody in C. elegans Protocol] |
| In Situ Hybridization Protocol - http://www.k-state.edu/hermanlab/protocols/in_situ_hybridization.htm Protocol for in situ hybridization. - [Read In Situ Hybridization Protocol] |
| Live Imaging of Caenorhabditis elegans: Examples - http://www.cshprotocols.org/cgi/content/extract/2006/29/pdb.ip19 To image early cleavages and chromatin dynamics, it is convenient to use histone H2B fused to GFP or lamin::GFP. Time-lapse movies can be obtained using conventional confocal microscope systems and their included software. Early embryos dissected from transgenic hermaphrodites are placed with egg salts on agar pads. - [Read Live Imaging of Caenorhabditis elegans: Examples] |
| Live Imaging of Caenorhabditis elegans: Observation of Nematodes and Data Collection Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/29/pdb.ip18 At low magnification, dissection microscopes are useful, whereas at higher magnification and resolution, a wide range of optical methods can be used, including differential interference contrast (DIC) optics, phase contrast, fluorescence, polarized light, confocal, and dark field. - [Read Live Imaging of Caenorhabditis elegans: Observation of Nematodes and Data Collection Protocol] |
| Live Imaging of Caenorhabditis elegans: Preparation of Samples - http://www.cshprotocols.org/cgi/content/abstract/2006/29/pdb.prot4601 Protocol describes useful techniques for preparing C. elegans for live microscopic analysis. Details of sample preparation depend on the developmental stage of the worm to be studied. - [Read Live Imaging of Caenorhabditis elegans: Preparation of Samples] |
| Preparation of Worm Extracts by Sonication Protocol - http://www.genetics.wustl.edu/tslab/Protocols/Cyto_extract_sonicat_v2.htm Protocol was designed to rapidly generate small scale cytosolic extracts of C. elegans for Western or IP (has not been tested for RNA work). The protocol works well for between 50 to 5000 worms and has not been extensively tested on larger a scale, though it should work. Includes: Collection; Sonication; Clearing lysate; Immunoprecipitation. - [Read Preparation of Worm Extracts by Sonication Protocol] |
| RNA In Situ Hybridization of Dissected Gonads with Digoxygenin-Labeled Probes Protocol - http://www.genetics.wustl.edu/tslab/Protocols/gonad_in_situ.html Protocol for RNA in situ hybridization of dissected gonads with digoxygenin-labeled probes. Includes: Preparation of single-stranded probes from cloned cDNA; Dissections and Fixation; Proteinase K digestion; Hybridization; Making a Mount. - [Read RNA In Situ Hybridization of Dissected Gonads with Digoxygenin-Labeled Probes Protocol] |
| Whole-Mount In Situ Hybridization for the Detection of RNA in C. elegans Embryos Protocol - http://cobweb.dartmouth.edu/~ambros/worms/14.html Protocol for whole-mount in situ hybridization for the detection of RNA in C. elegans embryos. Includes: Collection of Embryos; Permeabilization and fixation of embryos; Prehybridization; Hybridization; Post-hybridization washes; Probe Detection; Double-labeling; Interpretation. - [Read Whole-Mount In Situ Hybridization for the Detection of RNA in C. elegans Embryos Protocol] |
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