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Protocols and information related to bacterial cell culture media. Find protocols on cell culture media for agar and broth.
Agar Cell Culture Media Protocols (5)Broth Culture Media Protocols (4) |
| 2X YT Media (1000 ml) Protocol - http://www.thelabrat.com/protocols/2xyt.shtml Protocol for 2X YT Media Recipe (1000 ml). - [Read 2X YT Media (1000 ml) Protocol] |
| Expression Protocol in M9 Minimal Media via T7 Promoter - http://structbio.vanderbilt.edu/chazin/wisdom/labpro/M9.html This protocol has been used successfully to 15N or 13C/15N label our proteins using our pET1120/BL21(DE3) expression system: Preparing M9 minimal media begins with preparing a 5x stock solution of M9 salts. Generally, M9 salts contain a nitrogen source in the form of NH4Cl. Since we want to add a labeled nitrogen source, our 5x salts are prepared minus NH4Cl. Standard 5 X M9 Minimal Media salts minus nitrogen source For 1L 5xM9 salts: - [Read Expression Protocol in M9 Minimal Media via T7 Promoter] |
| M17 Media Protocol - http://www.thelabrat.com/protocols/M17Media.shtml Protocol for M17 media. - [Read M17 Media Protocol] |
| M9 Minimal Media (1000 ml) Protocol - http://www.thelabrat.com/protocols/m9minimal.shtml Protocol for M9 minimal media (1000 ml). - [Read M9 Minimal Media (1000 ml) Protocol] |
| NZ (200 Ml/500 Ml) Protocol - http://wheat.pw.usda.gov/~lazo/methods/lazo/stocksgl.html Protocol for NZ (200Ml/500Ml). - [Read NZ (200 Ml/500 Ml) Protocol] |
| NZCYM Media Protocol - http://www.thelabrat.com/protocols/NZCYM.shtml Protocol for NZCYM media. - [Read NZCYM Media Protocol] |
| NZM Media Protocol - http://www.thelabrat.com/protocols/nzm.shtml Protocol for NZM media. - [Read NZM Media Protocol] |
| NZYM Media Protocol` - http://www.thelabrat.com/protocols/nzym.shtml Protocol for NZYM media. - [Read NZYM Media Protocol`] |
| SOB Media Protocol - http://www.thelabrat.com/protocols/SOB.shtml Protocol for SOB media. - [Read SOB Media Protocol] |
| SOC Media (1000 ml) Protocol - http://www.thelabrat.com/protocols/15.shtml Protocol for SOC media (1000ml). - [Read SOC Media (1000 ml) Protocol] |
| YPD Media Protocol - http://www.thelabrat.com/protocols/YPDmedia.shtml Protocol for YPD media. - [Read YPD Media Protocol] |
| YPG Media Protocol - http://www.thelabrat.com/protocols/YPGmedia.shtml Protocol for YPG media. - [Read YPG Media Protocol] |