Find information and protocols on bacterial cell culture. Including bacterial cell culture media (agar, broth), Bacterial Plating, Bacterial Stocks, Fermentation Equipment and other bacterial cell culture related protocols and information.
Extraction and Solubilization of Total Protein from Microorganisms Protocol The growth conditions of microbial cell cultures and the time of sample collection should be optimized and standardized when growing cells for protein extraction. Because cells may excrete proteases and other extracellular enzymes, and compounds in the medium may interfere with extraction, wash cultures with an isotonic buffer, such as PBS or sucrose before solubilization.
Immunoprecipitation: Lysing Bacteria by Sonication Protocol In this protocol, bacterial cells are lysed by being subjected to short, intense treatments with ultrasound, which breaks the cell walls and shears the DNA into sizes that will not affect the viscosity of the samples. Note that this method causes some denaturation of the samples. The resulting lysate is ready for preclearing.