Bioinformatics, Protocols, DNA RNA Protein Proteomics
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Find information and protocols on bacterial cell culture. Including bacterial cell culture media (agar, broth), Bacterial Plating, Bacterial Stocks, Fermentation Equipment and other bacterial cell culture related protocols and information.
Bacterial Cell Culture Media Protocols (14)Bacterial Plating Protocols (2) | Bacterial Stocks Protocols (6)Fermentation Equipment (1) |
| Cell Mass by Measurement of Turbidity Protocol - http://homepages.gac.edu/~cellab/chpts/chpt12/ex12-5.html Protocol for measurement of cell mass by turbidity. - [Read Cell Mass by Measurement of Turbidity Protocol] |
| Examination of Bacterial Colonies Protocol - http://homepages.gac.edu/~cellab/chpts/chpt12/ex12-2.html Protocol for examination of bacterial colonies. - [Read Examination of Bacterial Colonies Protocol] |
| Extraction and Solubilization of Total Protein from Microorganisms Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/1/pdb.prot4224 The growth conditions of microbial cell cultures and the time of sample collection should be optimized and standardized when growing cells for protein extraction. Because cells may excrete proteases and other extracellular enzymes, and compounds in the medium may interfere with extraction, wash cultures with an isotonic buffer, such as PBS or sucrose before solubilization. - [Read Extraction and Solubilization of Total Protein from Microorganisms Protocol] |
| Immunoprecipitation: Lysing Bacteria by Sonication Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/23/pdb.prot4532 In this protocol, bacterial cells are lysed by being subjected to short, intense treatments with ultrasound, which breaks the cell walls and shears the DNA into sizes that will not affect the viscosity of the samples. Note that this method causes some denaturation of the samples. The resulting lysate is ready for preclearing. - [Read Immunoprecipitation: Lysing Bacteria by Sonication Protocol] |
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