Bioinformatics, Protocols, DNA RNA Protein Proteomics
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Nature composes some of her loveliest poems for the microscope and the telescope. ~Theodore Roszak, Where the Wasteland Ends, 1972
Protocols and information related to bacterial genetics.
| Bochner's Selection of Tets Protocol - http://rothlab.ucdavis.edu/protocols/bochner-selection.html Protocol for Bochner's selection of Tets. - [Read Bochner's Selection of Tets Protocol] |
| Hydoxylamine Mutagenesis of Plamid DNA Protocol - http://rothlab.ucdavis.edu/protocols/HA-mutagenesis.html Protocol for hydoxylamine mutagenesis of plasmid DNA. - [Read Hydoxylamine Mutagenesis of Plamid DNA Protocol] |
| Identification of Positive GATEWAY Expression Clones Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/29/pdb.prot4647 Protocol for the identification of positive GATEWAY expression clones when both the pENTRY and pDEST vectors contain the same marker for bacterial selection. Protocol describes ways in which difficult vector combinations can be used effectively to obtain the appropriate expression clone without having to convert the pENTRY clone or pDEST clone to vectors with compatible antibiotic resistances. - [Read Identification of Positive GATEWAY Expression Clones Protocol] |
| PCR Analysis of Agrobacterium Protocol - http://www.cshprotocols.org/cgi/content/abstract/2006/30/pdb.prot4667 Protocol describes a method to determine the presence of plasmid DNA in an Agrobacterium culture. Compared to selection of transformed Agrobacterium, which can be ambiguous and normally takes several days for resistant colonies to appear, the approach described here is both rapid and accurate. - [Read PCR Analysis of Agrobacterium Protocol] |
| Transposon-Mediated Mutagenesis Protocol - http://www.genome.wisc.edu/resources/protocols/tnmutationprotocol.htm Protocol for transposon-mediated mutagenesis. Includes: Amplify ORF from MG1655; Transposition reaction; Transformation; Picking; Verify mutation by Culture PCR; Confirm mutations by sequencing; Curing the temperature sensitive pKD46 plasmid. - [Read Transposon-Mediated Mutagenesis Protocol] |
| Triparental Mating Procedure for Xanthomonas (Recipient) Protocol - http://wheat.pw.usda.gov/~lazo/methods/lazo/xctripar.html Protocol for triparental mating procedure for Xanthomonas (recipient). - [Read Triparental Mating Procedure for Xanthomonas (Recipient) Protocol] |
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