Bioinformatics, Protocols, DNA RNA Protein Proteomics
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Protocols and information related to BACs, Bacterial Articficial Chromosome.
| A Triple Color FISH Technique for Mouse Chromosome Identification - http://info.med.yale.edu/genetics/ward/tavi/mamgenome.html Describe a set of BACs that map close to the centromeric and distal chromosomal ends for each mouse chromosome. - [Read A Triple Color FISH Technique for Mouse Chromosome Identification] |
| Amplification of Insert End Sequences from BACs Clones by Thermal Asymmetric Interlaced PCR - http://pubs.nrc-cnrc.gc.ca/ispmb/ispmb16/16175-2.pdf Protocol presents the amplification of insert end sequences from bacterial artificial chromosome clones using TAIL-PCR. The amplified products are suitable as probes for chromosome walking and genome mapping and as templates for direct sequencing. The protocol has been used in rice genome studies. - [Read Amplification of Insert End Sequences from BACs Clones by Thermal Asymmetric Interlaced PCR] |
| BAC-FISH Protocol - http://www.nhm.ac.uk/hosted_sites/schisto/protocols/BAC_FISH.html Protocol uses the BIOPRIME reaction kit from GibcoBRL to prepare biotin-labelled BAC DNA which is detected using FITC-Avidin (Vector Labs, DCS grade). Reagents from other manufacturers may work equally well but have not been tested. Includes: Labeling of BAC clones; Ethanol precipitation; Hybridization; Post-hybridisation treatment / detection. - [Read BAC-FISH Protocol] |
| BACs Bacterial Artificial Chromosomes - http://openwetware.org/wiki/Bacterial_artificial_chromosomes Information for BACs, Bacterial Artificial Chromosomes. Includes: Existing vectors; pBAC108L; pBeloBAC11; pBACe3.6; pFW11 and F' factor; pCC1BAC; pSMART VC Vectors; pIndigoBAC-5; Cosmids; pWEB-TNCTM; SuperCos 1; pFos1; PAC vectors; BAC purification; Putting the BAC into E. coli. - [Read BACs Bacterial Artificial Chromosomes] |
| DNA Isolation From BAC & PAC Clones Protocol - http://bacpac.chori.org/bacpacmini.htm Rapid alkaline lysis miniprep method for isolating DNA from large PAC clones. It is a modification of a standard Qiagen-Tip method that uses no organic extractions or columns. The method works very well for doing analytical restriction digests of PAC clones and can be scaled up if necessary. - [Read DNA Isolation From BAC & PAC Clones Protocol] |
| Dye Protocols and Notes for Cosmid, BAC, BAC, Fosmid Templates - http://www.genome.ou.edu/big_dyes_cos_bac_pac_fos.html Sequencing conditions tested for the ABI Big-Dye terminators (PE-ABI #4303150 for the 1000 reaction kit - Description: TF,KIT BTD RR-1000) with various templates. Important to quantitate all templates by agarose gel electrophoresis vs size and concentration standards and do a few tests with different template concentrations to determine the optimal conditions for your reactions. Several conditions are given. - [Read Dye Protocols and Notes for Cosmid, BAC, BAC, Fosmid Templates] |
| Isolating BAC DNA from Bacterial Cultures Using the NucleoBond System Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/16/pdb.prot4390 Protocol describes isolation of BAC DNA from bacterial cultures using the commercial NucleoBond system. - [Read Isolating BAC DNA from Bacterial Cultures Using the NucleoBond System Protocol] |
| Isolation of BAC DNA from Large-scale Cultures Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot4007 The procedure for isolation of BAC DNA is scaled-up to accommodate 500-ml cultures, which, on average, yield 20-25 µg of purified BAC DNA. - [Read Isolation of BAC DNA from Large-scale Cultures Protocol] |
| Isolation of BAC DNA from Small-scale Cultures Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot4006 BAC DNAs are prepared from 5-ml cultures of BAC-transformed cells by a modification of the standard alkaline lysis method (Preparation of Plasmid DNA by Alkaline Lysis with SDS: Minipreparation). The yield typically varies between 0.1 and 0.4 µg of BAC DNA. - [Read Isolation of BAC DNA from Small-scale Cultures Protocol] |
| Preparation of BAC DNA with CONCERT High Purity Plasmid Purification System Protocol - http://intramural.nimh.nih.gov/lcmr/snge/Protocols/BACProtocols/BACColumn.html Protocol for the preparation of BAC (Bacterial Artificial Chromosome) DNA with CONCERT high purity plasmid purification system. Includes: - [Read Preparation of BAC DNA with CONCERT High Purity Plasmid Purification System Protocol] |
| Purification of BAC DNA Prepared with the NucleoBond System Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/16/pdb.prot4391 Protocol describes purification of BAC DNA that has been prepared with the commercial NucleoBond system. - [Read Purification of BAC DNA Prepared with the NucleoBond System Protocol] |
| Working with Bacterial Artificial Chromosomes Protocol - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot4010 Protocol describes how to transform E. coli with BAC DNA by electroporation. - [Read Working with Bacterial Artificial Chromosomes Protocol] |
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