Thionin Staining

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Thionin Staining

Date Added: May 19, 2008 06:14:00 PM

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Category: Histology Protocols: Histology Staining Procedures

Thionin Staining

(gurr, 1958)

Fixation: 10% formalin or other general fixative.

Solutions:

Thionin: thionin, C.I. 52000, saturated aqueous 0.5 ml. Distilled water 100.0 ml. Molybdate-ferricyanide solution : ammonium molybdate, 5% aqueous 50.0 ml. Potassium ferricyanide, 1% aqueous 50.0 ml. Make up solutions fresh each time.

Procedure:

1. Deparaffinize and hydrate slides to water. See Acid Mucopolysaccharides under Saccharides, page 2G9. 11 Mast Cells 281

2. Stain in thionin: 5-15 minutes.

3. Rinse in distilled water.

4. Treat with molybdate-ferricyanide: 2 minutes.

5. Wash in distilled water: 2-3 minutes.

6. Dehydrate, clear, and mount. Results: acid mucopolysaccharides — purple other cell elements — bhiish

Stain Comments:

The molybdate-ferricyanide soliuion prevents loss of metachromasia. See Kiiyer {1911) for suggestions concerning fluorescent methods for mucopolysaccharides.

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