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Thionin Stain

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Protocols » Article Details

Thionin Stain
Date Added: May 19, 2008 06:10:57 PM
Author:
Category: Histology Protocols: Histology Staining Procedures

Thionin Staining Protocol



(mallory, 1938) fixation: any general fixative, but alcoholic preferred.

SOLUTION :

thionin, C.I. 52000 ................1.0 gm. 
25% ethyl alcohol ..............100.0 ml. 


Thionin Staining Procedure:



1. Deparaffinize and hydrate slides to water; remove HgClo.

2. Stain in thionin: 15 minutes to 1 hour.

3. Differentiate in 95% alcohol.

4. Dehydrate in absolute alcohol, clear, and moimt. 


Thionin Staining Analysis and Results:


mucin — light to dark red or purple Acid Mucopolysaccharides.
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