Toluidine Blue Stain

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Toluidine Blue Stain

Date Added: May 19, 2008 06:08:12 PM

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Category: Histology Protocols: Histology Staining Procedures

Toluidine Blue

(lillie, 1929)

Fixation: any general fixative, but alcohol preferred.

Solution: toluidine O, C.I. 52040 ..........2.0 gm.

Distilled water ........................100.0 ml.

Procedure for Toluidine Blue Staining:

1. Deparaffinize and hydrate slides to water; remove HgCU.

2. Stain in toluidine blue: 1 minute.

3. Wash in water: 2-3 minutes.

4. Dehydrate in acetone, 2 changes: 3-5 minutes each.

5. Clear in xylene and mount.

Toluidine Blue Stain Analysis Results:

mucin — reddish violet nuclei and bacteria — deep blue cytoplasm, fibrous tissue — bluish green bone — bluish green cartilage matrix — bluish violet muscle — light blue cell granules — blue violet hyalin and amyloid — bluish green

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