Chrysoidin Stain

Chrysoidin Stain

Chrysoidin Stain


(Harada, 1957) 

Fixation: any general fixative. 

solution: 

chrysoidin Y, C.I. 1 1270 ................0.5 gm. 

distilled water .........................100.0 ml. 

Staining Procedure:


1. Deparaffinize and run slides down to 80% alcohol; remove HgCU 
if present. 

2. Stain in chrysoidin: 5-10 minutes. 

3. Rinse in distilled water. 

4. Dehydrate, clear, and moimt. 

results: 

mast cell gTanules — deep brown to black 
other elements — yellowish 


Stain Comments:


Staining with chrysoidin can be preceded with hematoxylin or PAS. The author likes this method.