Mast Cell Staining Protocol

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Mast Cell Staining Protocol

Date Added: May 19, 2008 05:45:07 PM

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Category: Histology Protocols: Histology Staining Procedures

Mast Cell Staining

Mast cells are of common occurrence in connective tissue. Because of their cytoplasmic granules, however, staining methods for these cells have been included in this cytoplasmic element section. The specific staining of these granules is the primary means of identification of mast cells. Neutral Red (allen, 1960)

Fixation: 10% formalin solution:

neutral red, C.I. 50040 .............................. 0.5 gm.
50% ethyl alcohol................... 100.0 ml.

Procedure:

1. Deparaffinize and run slides down to 70% alcohol.
2. Stain in alum hematoxylin: 3 minutes.
3. Wash, blue, and wash.
4. Stain in neutral red: 10 minutes.
5. Differentiate in 70% alcohol: 2-10 minutes.
6. Dehydrate in 95% alcohol: 3 minutes.
7. Dehydrate in 7z-butyl alcohol, 2 changes: 5-10 minutes in each.
8. Clear and mount. Results: mast cell granules — red cartilage matrix — red nuclei — blue

Comments: Color is paler than with metachromatic methods

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