Purifying DNA Fragments from Agarose Gels Alternate Protocol

Purifying DNA Fragments from Agarose Gels Alternate Protocol

Step by Step Method for Purifying DNA Fragments from Agarose Gels

1. First, excise your band of interest from the agarose-TAE gel
2. Estimate the volume by weighing the gel slice on a scale.
3. Dissolve the agarose gel slice in 3 volumes of NaI (either make your own or obtain from a Geneclean kit) at 55oC for 5 minutes or more until the gel dissolves.
4. Add 1 ml of Wizard miniprep resin (Promega), 10 min. at room temperature.
5. Add the DNA resin mixture to a syringe and pass through a minicolumn.
6. Wash with 2 ml of Wizard wash buffer; spin 2 min in a microfuge.
7. Elute with 70oC TE or water (50 ul), 2 min in a microfuge.