Paraffin Embedding Protocol

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Paraffin Embedding Protocol

Date Added: March 02, 2008 10:35:10 PM

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Category: Histology Protocols

Paraffin Embedding Protocol

1. Fix tissue in 70% Ethanol (See Tip #1).

2. Place the tissue in the tissue processor. Process with the times and temperatures described in Image 1.

3. Embed the specimen in paraffin.

4. Attach the tissue block to a hardwood block by melting the back of the tissue block with a warm spatula and firmly pressing the two together.

5. Use a standard microtome to cut 8 μm thick sections (see Tip #2).

5. Store the sections and the paraffin blocks at or below room temperature.

Paraffin Embedding Buffer Recipes

70% (v/v) Ethanol

Tissue Processor		(Sakura FineTek Inc.) Sakura FineTek V.I.P.

Embedding Molds		super metal based 66 x 54 x 15 mm (Surgapath Med. Ind. Inc)

Xylenes		Reagent Grade

Paraffin Wax		(Paraplast; melting point 58°C)

100% (v/v) Ethanol

95% (v/v) Ethanol

80% (v/v) Ethanol

Paraffin Embedding Materials Needed

Paraffin
Ethanol
Xylenes

Paraffin Embedding Method Tips

1. Tissue processors in standard histology laboratories usually include Formalin-fixation as the first two steps in the paraffin infiltration procedure. It is important to avoid these steps when processing tissue intended for molecular profiling.

2. Microtome requires special instructions for usage. Please consult with personnel experienced in histology at your institution.

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