Fixation: any general fixative, but alcohol preferred.
Solution: toluidine O, C.I. 52040 ..........2.0 gm.
Distilled water ........................100.0 ml.
Procedure for Toluidine Blue Staining:
1. Deparaffinize and hydrate slides to water; remove HgCU.
2. Stain in toluidine blue: 1 minute.
3. Wash in water: 2-3 minutes.
4. Dehydrate in acetone, 2 changes: 3-5 minutes each.
5. Clear in xylene and mount.
Toluidine Blue Stain Analysis Results:
mucin — reddish violet nuclei and bacteria — deep blue cytoplasm, fibrous tissue — bluish green bone — bluish green cartilage matrix — bluish violet muscle — light blue cell granules — blue violet hyalin and amyloid — bluish green
