Thionin: thionin, C.I. 52000, saturated aqueous 0.5 ml. Distilled water 100.0 ml. Molybdate-ferricyanide solution : ammonium molybdate, 5% aqueous 50.0 ml. Potassium ferricyanide, 1% aqueous 50.0 ml. Make up solutions fresh each time.
Procedure:
1. Deparaffinize and hydrate slides to water. See Acid Mucopolysaccharides under Saccharides, page 2G9. 11 Mast Cells 281
2. Stain in thionin: 5-15 minutes.
3. Rinse in distilled water.
4. Treat with molybdate-ferricyanide: 2 minutes.
5. Wash in distilled water: 2-3 minutes.
6. Dehydrate, clear, and mount. Results: acid mucopolysaccharides — purple other cell elements — bhiish
Stain Comments:
The molybdate-ferricyanide soliuion prevents loss of metachromasia. See Kiiyer {1911) for suggestions concerning fluorescent methods for mucopolysaccharides.
