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Article Details
Preparing Stock Plating of Bacteria Protocol |
| Date Added: February 26, 2008 03:08:31 PM |
| Author: plinkadmin |
| Category: Bacterial Cell Culture Protocols: Bacterial Plating Protocols |
| Preparing a plating stock of bacteria to 200ml LB add 4 ml 10% Maltose (to = 0.2%) add either a single plaque or 1 ml of a pregrown bacterial strain (P2 for instance) shake at 300 rpm, 37oC overnight (or until you have sufficient growth) centrifuge at 4000 g for 10 min. bring up bacterial pellet in 80 ml (40% of original volume) of 10mM MgSO4 store at 4oC |
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