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Microinjection Pipettes Protocol

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Protocols » Article Details

Microinjection Pipettes Protocol
Date Added: March 02, 2008 09:44:35 PM
Author:
Category: Stem Cell Protocols

Glass Capillary Tubing Used for Microinjection


The glass capillary tubing commonly used for microinjection used should be thin walled, borosilicate glass without a fiber. (a good example is supplied by Clark Electromedical Instruments with the following properties:)
  • OUTSIDE DIAMETER: 1.0 mm
  • INSIDE DIAMETER: 0.78 mm
  • LENGTH: 150 mm
  • GC 100T to 15 (= 15 cm)
  • GC 100T to 10 (= 10 cm)


Note that micro-injection pipettes should have an internal diameter only marginally larger than the stem cells (18 to 20 μm) and a sharp point that allows the needle to penetrate cleanly in the blastocoel cavity.
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