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Lambda Phage Protocol for Large Preparation DNA Recovery

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Lambda Phage Protocol for Large Preparation DNA Recovery
Date Added: February 26, 2008 03:13:21 PM
Author: plinkadmin
Category: DNA Protocols
Protocol for Lambda phage DNA - Recovering DNA from Large Maxi Preparations Collect phage from SM dialysis add EDTA to equal 20mM add Proteinase K to equal 50 ug/ml. add SDS to equal 0.5% incubate at 65oC for 1 hr extract with equal volume phenol extract with equal ratio phenol/chloroform to total equal volume extract with equal volume chloroform dialyze against 1X T.E. overnight or longer
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Average rating: 8 (1 votes)
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admin: 2008-03-02 13:50:38
A simple great protocol

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