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Constructing Random-Peptide Libraries for Phage Display

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Constructing Random-Peptide Libraries for Phage Display
Date Added: April 11, 2008 09:14:09 PM
Author: plinkadmin
Category: Peptide Protocols: Phage Display Cloning Systems and Protocols
The library requires approximately 100 μg of cut vector with a roughly equimolar amount of degenerate insert in a total volume of 20 ml, followed by electroporation into electrocompetent cells. Amplification of an already existing library is described in detail in the protocol on library amplification.
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