Bismarck Brown Stain

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Bismarck Brown Stain

Date Added: May 19, 2008 05:53:11 PM

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Category: Histology Protocols: Histology Staining Procedures

Bismarck Brown Stain

(spatz, 1960)

Fixation: 10% formalin.

Solution: Bismarck brown: Bismarck brown, C.I. 21000......................... 0.5 gm.

Absolute ethyl alcohol .....................80.0 ml.
1% aqueous hydrochloric acid ........................20.0 ml.

Bismarck Brown Stain Procedure:

1. Deparaffinize and transfer sections to absolute alcohol: 3 minutes.

2. Dip in 957o. Then 70% alcohol.

3. Stain in Bismarck brown: 30 to 90 minutes.

4. Differentiate in 3 changes 70% alcohol: 2 seconds each.

5. Transfer to 95% alcohol: 2 seconds.

6. Dehydrate in absolute alcohol: 30 seconds.

7. Clear in xylene: 1 second; moiuit. Results: mast cell granules — yellow brown

Staining Comments:

If counterstaining is desirable, follow the 70% differentiation by 3 miniues in hematoxylin (Harris, Mayer's, etc.); dip in 70% alcohol: 2 seconds, and continue into step 5 above.

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