Mouse Genotyping Protocols

Practical Approach Online, Oxford University Press. Combined LacZ staining and in situ hybridization of mouse embryos, beta-galactosidase. - [Read Combined LacZ staining and in situ hybridization PDF]

Test mice and rats for the presence of the transgene by PCR. University of Michigan, Transgenic Animal Model Core - [Read Genotyping Transgenic Rodents by PCR]

PCR screens must be designed to detect transgene DNA at the single copy level.Copy standards are prepared by mixing non-transgenic tail DNA with a known amount of transgene DNA to produce transgene copy standards. University of Michigan Transgenic Animal - [Read reparation of Copy Standards for Southern Blot Copy Number Determination]