Protocol for JNK Kinase Assay Protocol describing a JNK kinase asssay. Protocol includes information on: Triton Lysis Buffer, Kinase Assay Buffer, Kinase Assay and Preparation of kinase
Raf-1 Kinase Assay This protocol uses mitogen-activated protein/ERK kinase (MEK) to activate the extracellular-signal-regulated (Erk) mitogen-activated protein (MAP) kinases upon agonist binding to receptors. Protocol includes information about: Harvest, Immunoprecipitation and Kinase Reaction. Also includes following solutions: Laemmli Sample Buffer (1X), ATP Mix, Kinase Buffer (10X), Lysis Buffer. Helpful protocol hints are also included.
SAPK/Jun Kinase Assays Protocol for SAPK/Jun kinase assays. Protocol includes information on: preparation of cell lysate, substrate/antibody, lysate interaction.