Cat Assays Protocols

Chloramphenicol acetyltransferase or CAT reporter gene and protein. CAT is a bacterial gene that evolved to neutralize the antibiotic chloramphenicol. To neutralize it, CAT transfers acetyle groups to chloramphenicol and thus changes its shape into a harmless form. Molecular biologists use CAT as a reporter protein in eukaryotic cells.

Protocols » Reporter Gene Assays » Cat Assays Protocols

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CAT Assay (Liquid Phase) Protocol - http://snbworld.com/cat2.html

Protocol describing a CAT assay (liquid phase). - [Read CAT Assay (Liquid Phase) Protocol]

CAT Assay Protocol - http://kinase.uhnres.utoronto.ca/methods/CATassay.html

Protocol for CAT assay. - [Read CAT Assay Protocol]

CAT Assay Protocols - http://www.biochem.northwestern.edu/ibis/morimoto/research/Protocols/VI.%20Transcription/G.%20CAT%20ASSAY.pdf

Two protocols describing CAT assays. - [Read CAT Assay Protocols]

Measurement of CAT in Extracts of Mammalian Cells Using Thin-layer Chromatography - http://www.cshprotocols.org/cgi/content/extract/2006/2/pdb.prot3951

In this protocol, extracts prepared from cells transfected with a chloramphenicol acetyltransferase (CAT) reporter plasmid are incubated with radiolabeled chloramphenicol. The acetylated products generated by the action of CAT are separated from the unmodified drug by thin-layer chromatography and quantitated by scraping the spots from the thin-layer plates and counting them by scintillation spectroscopy. - [Read Measurement of CAT in Extracts of Mammalian Cells Using Thin-layer Chromatography]