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Molecular Station is THE largest network of researchers, scientists and science lovers anywhere!
The saddest aspect of life right now is that science gathers knowledge faster than society gathers wisdom. ~Isaac Asimov, Isaac Asimov's Book of Science and Nature Quotations, 1988
2-dimensional gel electrophoresis, 2-D gel electrophoresis protocols and methods.
| 2-D Polyacrylamide Gel Electrophoresis (Prostate Tissue) - http://cgap-mf.nih.gov/Protocols/DNARNAProteomicAnalysis/Proteomics/2DPage.html A detailed protocol for 2-D Polyacrylamide Gel Electrophoresis using Prostate Tissue. Molecular Profiling Initiative. - [Read 2-D Polyacrylamide Gel Electrophoresis (Prostate Tissue)] |
| 2D Gel Electrophoresis for Human Plasma Proteome - http://web.archive.org/web/20031115200452/http://iprotocol.mit.edu/protocol/362.htm 2D Gel Electrophoresis for Human Plasma Proteome. Lee Lab. - [Read 2D Gel Electrophoresis for Human Plasma Proteome] |
| 2x Lysis Buffer Recipe - http://utzlab.stanford.edu/publications/lysisbuffer.doc 2x Lysis Buffer Recipe. Utz Lab. - [Read 2x Lysis Buffer Recipe] |
| Analysis of Proteins using Small Format 2D Gel Electrophoresis - http://www.abdn.ac.uk/~mmb023/protocol.htm Analysis of Proteins using Small Format 2D Gel Electrophoresis. Cash Lab - [Read Analysis of Proteins using Small Format 2D Gel Electrophoresis] |
| Detailed 2D Gel Electrophoresis - http://www.purdue.edu/dp/bbc_labs/2d.html Excellent Detailed Protocol for 2-D Gel Electrophoresis including staining, gel running, and equipment. Purdue PSAL. - [Read Detailed 2D Gel Electrophoresis] |
| In-Gel Digestion of Proteins Separated by Polyacrylamide Gel Electrophoresis - http://web.archive.org/web/20010906052645/http://www.pharma.ethz.ch/bmm/protocols/ingeldig.html In gel trypsin digestion for Mass spectrometry. (According to EMBL Protein and Peptide Group, 1997) - [Read In-Gel Digestion of Proteins Separated by Polyacrylamide Gel Electrophoresis] |
| Technical information on 2-D PAGE - http://us.expasy.org/ch2d/protocols/ Very Concise Protocol for 2D Electrophoresis. ExPASY. - [Read Technical information on 2-D PAGE] |