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Nuclear Extraction Protocols. Extraction methods and procedures for nuclear proteins from the nucleus.
Homogenize cells or tissue, Pellet nuclei, Lyse nuclei, Reprecipitate nuclear proteins, dialyze nuclear extract. Hattori M et al., DNA Cell Biol. 1990. Homogenization buffer and dialysis buffer recipes. - [Read Large scale nuclear extract preparation]
S100 extract cytosolic extraction preparation. Buffer A (Hypotonic Buffer), Low and High salt buffer recipes. Brent Graveley - [Read Nuclear and Splicing Extract Preparation]
Nuclear Extract protocol is optimized for about 10^7 cells, can use for gel shift assays and western blotting. Mirmira Lab, Univ. Virginia - [Read Preparation of Nuclear Extracts for Gel Shifts and Westerns Blots]
Nuclear Extract preparation protocols, Buffers and solutions for nuclear extracts. Hahn Lab - [Read Yeast Nuclear Extract (Small Scale)]
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