Arabidopsis Transformation Protocols

Electrotransformation of Agrobacterium with a plasmid that has been replicating in E. coli. Growth of Arabidopsis thaliana. Arabidopsis dunking. Seed Harvesting. Plant tissue culture. Very detailed protocol. Stockinger lab. PDF - [Read Arabidopsis transformation with Agrobacterium PDF]

Arabidopsis transformation and selection on kanamycin agar, Preparation of plants for transformation, Agrobacterium, Dipping, Post-dip care, Agar plates, Tips. Adaptation of the short protocol described bySteve Clough and Andrew Bent, University of Illinois at Urbana-Champaign. - [Read Arabidopsis transformation with pictures]

Protocol for a rapid and robust method of identifying transformed Arabidopsis thaliana seedlings following floral dip transformation. - [Read Identifying Transformed Arabidopsis thaliana Seedlings Following Floral Dip Transformation Protocol]

The plant transformation procedures described involve floral dip, vacuum infiltration, and spraying. They yield transformants at frequencies ranging up to several percent, with the most common frequency being 0.1%-1%. - [Read In Planta Transformation of Arabidopsis Protocols]

Protocol for an improved method for preparing Agrobacterium cells that simplifies the Arabidopsis transformation protocol. - [Read Preparing Agrobacterium Cells That Simplifies the Arabidopsis Transformation Protocol]

Arabidopsis can be stably transformed using Agrobacterium tumefaciens-mediated transfer of T-DNA. We describe the generation of transgenic plants via root transformation in tissue culture, which can be useful for transforming sterile mutants. - [Read Root Transformation of Arabidopsis Protocol]

Protocol for a simplified Arabidopsis transformation. Found that the MS salts, hormone, etc. make no difference, that OD of bacteria doesn't make much of a difference, that vacuum doesn't even make much of a difference as long as you have a decent amount of surfactant present. Plant health is still a major factor - healthy fecund plants make a big difference! With this method you should be able to achieve transformation rates above 1%. - [Read Simple Arabidopsis Transformation Protocol]

Agrobacterium tumefaciens strain transformation of arabidopsis thaliana. Steve Clough and Andrew Bent, University of Illinois at Urbana-Champaign. - [Read Simplified Arabidopsis Transformation Protocol]

Transformation Protocol for Arabidopsis Abbreviated. Bioforge - [Read Transformation Protocol for Arabidopsis Abbreviated]

Protocol for vacuum-infiltration transformation of Arabidopsis thaliana. Includes: Plant Growth; Vacuum Infiltration; Selection of Putative Transformants; Kanamycin selection. - [Read Vacuum-Infiltration Transformation of Arabidopsis thaliana Protocol]

A. tumefaciens is a soil-dwelling bacterium that transforms normal plant cells into tumor-forming cells by inserting a piece of bacterial DNA (the transfer, or "T," DNA) into the plant cell genome. The Ti plasmid also carries many of the transfer functions for mobilizing the T-DNA. This article provides a brief discussion of the principles of T-DNA transformation, including consideration of T-DNA vectors and their hosts. - [Read Vectors and Agrobacterium Hosts for Arabidopsis Transformation Protocol]