Yeast 2-Hybrid Protocols, procedure, and methods. Library, Bait, vector, yeast two hybrid screen for protein-proteni interactions, transformation.
2 Hybrid System TRAFO Protocol Yeast Two-Hybrid System. TRAFO Solutions. Gietz Lab
EXAMINING THE FUNCTION OF PROTEINS AND PROTEIN NETWORKS WITH THE YEAST TWO-HYBRID SYSTEM Interactor Hunt Protocol. Testing whether a bait activates transcription. Screening a library for interactors. Finley Lab. 1997.
GST Fusion Protein Prep Detailed protocols and methods for GST protein purification. Vesicle Trafficking, Stanford.
GST Fusion Protein Purification from Yeast GST Fusion Protein Purification from Yeast. Lysis Buffer recipe for yeast, glutathione bead method for GST purification. Dohlman Lab
LiOAc Yeast Transformation Protocol LiOAc/TE, 40% PEG in LiOAc/TE. Transformation Method and Protocol for Yeast. Finley Lab, Wayne State.
Two-hybrid analysis of genetic regulatory networks Finley and Brent. Excellent Two-Hybrid Resource Article and Protocols. Very Detailed.
Yeast Two Hybrid Assay: A Fishing Tale Theory, Galactose metabolism, The Pros and Cons of the Two hybrid Assay. Troubleshooting and Tips for Hybrid assay. Sobhanifar BioTeach Journal 2003.
Yeast Two-Hybrid Screen with Library and Bait yeast transformed with bait vector. yeast culture. selection plates. Mirmira, Univ. Virginia.
Yeast Two-Hybrid: State of the Art Yeast Two-Hybrid: State of the Art. Paper on Protein-protein interactions and the two-hybrid protocol. Wim Van Criekinge et al., BPO.
DNA Protocols - DNA Cloning
Cell Biology Protocols - Cell Culture Protocols
PCR Protocols Protein Protocols
Western Blot Protocols
For science news click here:Science News