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Protocols

Molecular Station Protocols Online Database for DNA, RNA, Molecular Biology, Protein, Proteomics, and Molecular Model Organisms: Protocols, Techniques, and Procedures.

Protocols

Welcome to the protocols area. Protocols are categorized by section and each section contains sub-categories. You may also search for the method you need in the searchbar.

Animal Research Techniques (13)

Antibody Microarray Protocols (8)

Antibody Protocols (4)

Antibody Production Protocols, Antibody Handling Protocols, Epitope Mapping Antibody Protocols, Antibody Labeling Protocols, ...

BACs Bacterial Artificial Chromosome Protocols (12)

Bacteria Genetics Protocols (6)

Bacteria Transformation Protocols (5)

Bacterial Cell Culture Protocols (8)

Bacterial Cell Culture Media Protocols, Bacterial Plating Protocols, Fermentation Equipment, Bacterial Stocks Protocols, ...

Buffers Media, and Solutions (18)

Bacterial Solutions, Buffer Theory, Buffer Recipes for Protein Work, Buffer Recipes for DNA Work, Buffers for RNA Work, ...

C. elegans Protocols (17)

C. elegans Fixing Protocols, C. elegans Injection Protocols, C. elegans Staining Protocols, C. elegans Genetics Protocols, C. elegans Cell Culture Protocols, ...

Cell Biology and Cell Culture (66)

ES Stem Cell Biology and Culture, Apoptosis Protocols, Cell Culture Techniques, Troubleshooting Cell Culture Problems, Cell Culture Growth Media, Cell Transfection Protocol, Cell Electroporation Protocols, Cell Line Culture Conditions, Cell Lysis Protocols, Cell Cycle Protocols, Cell Adhesion Protocols, Hepatocyte Cell Culture, Epithelial Cell Culture, In Vitro Reconstitution Protocols, Cell Viability Protocols, ...

Cell Culture Protocols (20)

Cell Culture Contamination Prevention and Removal Protocols, Cell Immortalization Protocols, Cell Lysis Protocols, Cell Storing and Cell Freezing Protocols, Trypsinization Protocols, Cell Fixing Protocols, Cell Lines Protocols, Cell Staining Protocols, Sterile Technique Protocols, Insect Cell Culture Protocols, ...

Cell Immortalization Protocols (1)

Cell Organelle Protocols (6)

Extracellular Matrix Protocols, Microsome Protocols, Nucleus Protocols, Lysosome Protocols, Mitochondria Protocols, Subcellular Fractionation Protocols, ...

Chromatography Protocols (16)

DNA RNA Affinity Chromatography, Immunoaffinity Chromatography Protocols, Column Chromatography Protocols, Gel Filtration Chromatography Protocols, Dye Ligand Affinity Chromatography Protocols, Immobilized Metal Ion Affinity Chromatography Protocols, Affinity Chromatography Protocols, Gas Chromatography Protocols, Ion Exchange Chromatography Protocols, Liquid Chromatography Protocols, ...

Cloning Protocols (10)

Baculovirus Protein Expression Protocols, Mutagenesis Protocols, Vector Protocols, ...

Cytogenetics Protocols (38)

Comparative Genomic Hybridization CGH Protocols, Array CGH, Fluorescent In Situ Hybridization FISH Protocol, DIG Biotin Labeled Probe Protocols, In Situ Hybridization Tissue Sections Protocols, RNA In Situ Hybridization Protocols, Spectral Karyotyping SKY Protocols, In Situ Hybridization Protocols, ...

Cytoskeleton Protocols (3)

Actin Myosin Protocols, Intermediate Filaments Protocols, Microtubules Protocols, ...

Developmental Biology (4)

Sea Urchin Developmental Biology, Embryology Protocols, Utilizing Model Organisms Protocols, ...

DNA Microarray Protocols (27)

DNA Methylation Microarray CpG, DNA Microarray Software, Troubleshooting DNA Microarray, ...

DNA Protein Interactions Protocols (6)

Dnase Footprinting Assay Protocols, Electrophoretic Mobility Shift Assays EMSA Protocols, ...

DNA Protocols (19)

EMSA DNA-Protein Protocols, PCR Polymerase Chain Reaction Protocols, DNA Paternity Testing, DNA Cloning, DNA Extraction Protocols, Chromatin Immunoprecipitation Protocols, DNA Extraction Blood, TOPO Cloning, TOPO TA Cloning, SAP Protocols, T4 Polynucleotide Kinase Protocols, SNP Protocols, cDNA Library Protocols, Genomic DNA Library Protocols, Cosmid Library Protocols, ...

Drosophila Protocols (14)

Drosophila Dissection Protocols, Drosophila Staining Protocols, Drosophila Genetics Protocols, Drosophila Culture Media Protocols, Drosophila Nucleic Acids Protocols, ...

E Coli Protocols (4)

E coli Genetics Protocols, E coli Protein Protocols, E coli Nucleic Acid Protocols, E coli Transformation Protocols, ...

Electrophysiology Ion Channels (4)

ELISA Protocols (23)

Sandwich ELISA Assay Protocols, ELISpot Protocols, ...

Epigenetics and Methylation Protocols (10)

5-Aza-dC Treatment AZA Procedure, Bisulfite Treatment Methylation Assay Protocol, ChIP Chromatin Immunoprecipitation Protocols, ...

Fluorescent Dyes Protocols (2)

Fungi Protocols (11)

Aspergillus Protocols, Neurospora Protocols, Fungi Genetics Protocols, ...

Gene Delivery Protocols (3)

Biolistics Protocols, DNA Nanoparticles Protocols, ...

General Research Protocols and Methods (11)

Centrifuge Use and Centrifugation, Laboratory Safety, Spectrophotometry Protocols, Sterile Technique, ...

Genetics and Genomics (2)

Genotyping Protocols, Cancer Genetics Protocols, ...

Histology Protocols (7)

Histological Fixation, Histology Staining Procedures, Fixation of Cells Tissues Protocol, Laser Capture and Microdissection, Tissue Sample Sectioning, ...

Histology Stains Protocols (13)

Amine Stains Protocols, Connective Tissue Stains Protocols, Giemsa Stains Protocols, Melanin Stains Protocols, Nucleic Acid Stains Protocols, Bacterial Stains Protocols, Enzyme Histochemical Stains Protocols, Hematoxylin Eosin Staining Protocols, Mucin Stains Protocols, ...

Immunohistochemistry Protocols (27)

Antibody Staining Protocols, Blocking Protocols, Cryosectioning Protocols, Resin Staining Protocols, Antigen Retrieval Protocols, Coated Treated Slides Protocols, Fixation Protocols, ...

Immunology (22)

Antigen Retrieval, Immunocytochemistry Protocols, Chemotaxis, Immunoassays, Antigen, Macrophage Monocyte Protocols, Mononuclear Cell Isolation Protocols, T Cell Protocols, B Cell Protocols, Immunofluorescence Protocols, Immunoprecipitation Protocols, ...

Laboratory Model Organisms Protocols (7)

Luciferase and Dual Luciferase Assays (4)

Mass Spectrometry Protocols (22)

Microarray Protocols (5)

Amino-allyl Microarray Methods, Microarray Chip Protocols, Microarray DNA Labeling Protocols, Microarray Hybridization Protocols, Microarray Analysis Protocols, ...

Microbiology (5)

Bacterial Methods E.Coli Protocols, Bacterial Competent Cells Preparation E.Coli, Antibiotics Concentration in Media LB, Bacterial E.Coli Culture Media & Plates, Mini-Prep Plasmid Purification, ...

Microinjection Protocols (5)

Microinjection Equipment, Microinjection of Proteins Protocols, Microinjection of DNA Protocols, Microinjection Setup Protocols, ...

Microscopy Protocols (10)

Confocal Microscopy Protocols, Fluorescence Microscopy Protocols, In Vivo Imaging Protocols, Optical Microscopy Protocols, Electron Microscopy Protocols, Live-Cell Imaging Protocols, ...

Microsphere Analysis Protocols (2)

Molecular Biology Protocols (10)

DNA Ligation Protocols, Restriction Enzyme Digestion, Phage Protocols and Methods, Agarose Gel Preparation, Carbohydrate Protocols, ...

Molecular Imaging (15)

Confocal Microscopy Protocols and Information, Light Microscopy, Confocal Microscope Protocols, Electron Microscopy Protocols, Immunofluorescence Microscopy, ...

Molecular Model Organisms (7)

Arabidopsis Thaliana, Yeast Two-Hybrid Screen Protocol, Mouse Animal Protocols, ...

Mouse Protocols (17)

Assembling Chimeras Mouse Protocols, Mouse Embryology Protocols, Mouse Surgical Procedures Protocols, Mouse Reproductive System Surgery Protocols, Mouse Organ Removal Surgery Protocols, Mouse Cell Culture Protocols, Mouse Genetics Protocols, ...

Neuroscience Protocols (10)

Histology Stains Protocols, Neuronal Cell Culture Protocols, Patch Clamping Protocols, Neuronal Stains Protocols, ...

Nucleic Acid Detection Protocols (7)

Nucleic Acid Blotting Protocols, ...

Nucleic Acid Electrophoresis Protocols (8)

DNA Electrophoresis Protocols, RNA Electrophoresis Protocols, Pulsed Field Gel Electrophoresis Protocols, ...

Nucleic Acid Modification Protocols (5)

DIG Biotin Labeled Probes Protocols, Nucleic Acid Radiolabeling Protocols, Random Primer Labeling Protocols, Nucleic Acid Labeling Protocols, DNA Methylation Protocols, ...

Nucleic Acid Purification Protocols (12)

DNA Isolation Protocols, RNA Isolation Protocols, ...

Oligonucleotide Protocols (1)

Oligonucleotide Purification Protocols, ...

PCR Protocols (49)

PCR Optimization, Standard PCR Protocol, PCR Troubleshooting, Colony PCR, Long PCR Protocol. Extended PCR., In-situ PCR Protocols, AFLP PCR, Bisulfite Conversion Based PCR Protocols, PCR Cloning Protocols, Quantitative PCR Protocols, Real-Time PCR Protocols, cDNA Synthesis Protocols, Inverse PCR Protocols, PCR Buffer Protocols, Reverse Transcriptase RT-PCR Protocols, ...

Peptide Protocols (6)

Antibodies from Peptides Protocols, Phage Display Cloning Systems and Protocols, Dissolving Handling Peptides, Peptide Storage, ...

Pharmacology and Toxicology (0)

Pharmacology and Toxicology Protocols (7)

Cytotoxicity Protocols, Drug Metabolism Protocols, Receptor Binding Protocols, Drug Discovery, Enzyme Assays Protocols, Pharmacokinetics Protocols, ...

Plant Biology Protocols (23)

Plant PCR, Arabidopsis Tissue Culture, Arabidopsis Transformation Protocols, Plant Tissue Culture, Plant DNA RNA Isolation Protocols, Plant Genetics Protocols, Photosynthesis and Respiration Protocols, Plant Proteins, Peptides and Antigens, Arabidopsis Genetics Protocols, Arabidopsis Protein Peptide Protocols, Arabidopsis Nucleic Acid Protocols, ...

Primary Cell Isolation and Culture Protocols (13)

Laser Capture Microdissection Protocols, Mammalian Cell Culture Protocols, ...

Protein Biochemistry Protocols (0)

Protein Microarray Protocols (8)

Protein Protocols (24)

Western Blot Protocol, Immunoprecipitation Protocols, Protein Purification and Identification, Protein Precipitation Procols, Nuclear Extraction Protocols, Protein Phosphorylation Protocols, Protein Expression Protocols, Co-immunoprecipitation, Protein Electrophoresis, Protein Extraction Protocols, Protein Extraction From Cells, Protein Extraction From Tissue, Protein Dialysis Protocols, Antibody Purification Protocols, Protein Ubiquitination Protocols, ...

Protein Staining Protocols (3)

Proteomic Protocols (15)

2-D Gel Electrophoresis, Silver Staining Protocols, ...

Radioactivity (5)

Radioaction Safety, Autoradiography, Liquid Scintillation Counting, ...

Recombinant Protein Protocols (5)

Baculovirus Recombinant Protein, ...

Reporter Gene Assays (8)

Beta-galactosidase Assay Enzyme LacZ, Cat Assays Protocols, GFP Assay Protocols, Luciferase Assay Protocols, B-Galactosidase Assay Protocols, ...

RNA Protocols (11)

RNA-binding Protein Purification, cDNA Libraries Library, RT-PCR and cDNA synthesis, RNA-binding Protein EMSA Interactions, 3' RACE Rapid Amplification of cDNA Ends Protocols, 5' RACE - Rapid Amplification of cDNA Ends Protocols, In Vitro Transcription T7 SP6 T3 Protocols, rRNA Extraction and Isolation, mRNA Extraction Protocols, ...

RNAi Technology Protocols (15)

RNAi C. elegans Protocols, RNAi Mammalian Cells Protocols, RNAi Avian Embryos Protocols, RNAi Drosophila Protocols, RNAi Mouse Embryos and Oocytes Protocols, ...

Signalling Signal Transduction Protocols (29)

Calcium Measurement Protocols, Chemokine Protocols, Cytokine Protocols, MAPK Protocols, Protein Kinase Protocols, ...

Staining Protocols (2)

Gram Staining Protocols, Simple Staining Protocols, ...

Stem Cell Protocols (14)

Stem Cell Isolation Protocols, Stem Cell Culture Protocols, Feeder Cell Preparation Protocols, Mouse Embryonic Fibroblasts Protocols, Stem Cell Injection Protocols, ...

Tetrahymena Protozoa Protocols (8)

Transfection Protocols (13)

Calcium Phosphate Transfection Protocols, Transient Transfection Protocols, Stable Transfection Protocols, Electroporation Sonoporation Protocols, ...

Transgenic Mouse Protocols (5)

Mouse Genotyping Protocols, Mouse Tail DNA Extraction Genotyping, ...

Viral Culture and Isolation Protocols (2)

Viral Isolation Protocols, Viral Culture Protocols, ...

Viral Manipulation Protocols (3)

Phage Protocols, M13 Phage Manipulation Protocols, Phage Lamda Manipulation Protocols, ...

Western Blot Protocols (8)

Antigen Detection Methods Protocols, Immunoblot Stains for Total Protein Protocols, General Western Blot Methods Protocols, Immunoblot Stripping Protocols, ...

Xenopus Protocols (10)

Xenopus Embryo Manipulation Protocols, Xenopus Injection Protocols, Xenopus Egg Extracts Protocols, Xenopus Genetics Protocols, ...

Yeast Cell Culture Protocols (2)

Yeast Media, Solutions and Stocks Protocols, Yeast Sporulation Protocols, ...

Yeast Protocols (17)

Yeast Genetics Protocols, Yeast Nucleic Acid Protocols, Yeast Protein Protocols, Yeast Transformation Protocols, Yeast Cell Synchrony Protocols, Yeast Staining Protocols, Yeast Two-Hybrid Systems Protocols, ...

3' Rapid Amplification of cDNA Ends RACE Using PCR Protocol. This protocol contains the steps for 3' end rapid amplification of mRNA by PCR. The first-strand cDNA is synthesized from total or poly(A+) RNA by priming from the poly-A tail of the mRNA using a oligo (dT) adaptor primer. The cDNA is then amplified via PCR using a gene-specific primer and an adaptor primer.

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